Preliminary evaluation of mast cells and angiogenesis processes in experimental fibrosarcoma.

Abstract

The aim of the study was the evaluation of angiogenesis processes in fibrosarcoma induced by 3-methylcholanthrene (3-Mc) in reference to the number of mast cells (MCs). 76 male Wistar rats were divided into 4 groups: two experimental (E) groups--after injection of 0.2 mg 3-Mc dissolved in olive oil (0.25ml), and 2 control (C) groups. In E1 group, 52 rats were killed after development of the fibrosarcoma; E(2)--10 rats were killed before development of the tumor; C(1)-8 rats received 0.25ml olive oil; C(2)--8 rats received no treatment. Tissue material was fixed in buffered formalin or Carnoy's and Bouin's fluid. Paraffin sections were stained with H+E and Azan methods, and with alcian blue-saphranine and toluidine blue. Immunohistochemical reactions detecting tryptase in MCs were also performed. Angiogenic objects (microvessels and single endothelial cells) were recognized using antibodies against factor VIII (vWF), P selectin (CD-62P), and CD-90. We found a distinct relationship between intensification of neoangiogenesis at the tumor periphery and increased number of MCs.

• Authors: B Sawicki, I Kasacka, L Chyczewski
• Journal: Folia histochemica et cytobiologica (Folia Histochem Cytobiol) Vol. 40 Issue 2 Pg. 197-8 ( 2002) ISSN: 0239-8508 [Print] Poland
• PMID: 12056643 (Publication Type: Journal Article)

Chemical References

• Biomarkers, Tumor
• Carcinogens
• Methylcholanthrene
• Serine Endopeptidases
• Tpsab1 protein, rat
• Tpsb2 protein, rat
• Tryptases

Topics

• Animals
• Biomarkers, Tumor (analysis)
• Carcinogens
• Fibrosarcoma (blood supply, chemically induced, pathology)
• Immunohistochemistry
• Male
• Mast Cells (pathology)
• Methylcholanthrene
• Neovascularization, Pathologic (pathology)
• Rats
• Rats, Wistar
• Regional Blood Flow (physiology)
• Serine Endopeptidases (metabolism)
• Staining and Labeling
• Tissue Embedding
• Tryptases