Ectopic ACTH syndrome: discrepancy between somatostatin receptor status in vivo and ex vivo, and between immunostaining and gene transcription for POMC and CRH.

Abstract

OBJECTIVE:

To characterize somatostatin receptor status in vivo and ex vivo and immunoreactivity and gene transcription for proopiomelanocortin (POMC) and corticotropin-releasing hormone (CRH) in a case of Cushing's syndrome caused by a sporadic metastatic medullary thyroid carcinoma (MTC).

METHODS:

(111)In octreoscan, analysis of tumorous mRNA transcripts for somatostatin receptor subtypes (SSTR) as well as for POMC and CRH.

RESULTS:

The tissue was intensely positive by (111)In octreoscan but expressed only SSTR 1, 3 and 5. There was immunopositivity only for CRH, but gene transcription for both POMC and CRH was seen.

CONCLUSIONS:

(1) This first comparison between somatostatin receptor status in vivo and ex vivo in MTC shows a marked positive octreoscan despite absent SSTR 2 expression, and (2) this is the first report of a discrepancy between immunostaining and gene transcription for POMC and CRH.

• Authors: Mikkel T Kristiansen, Lars M Rasmussen, Niels Olsen, Sylvia L Asa, Jens O L Jørgensen
• Journal: Hormone research (Horm Res) Vol. 57 Issue 5-6 Pg. 200-4 ( 2002) ISSN: 0301-0163 [Print] Switzerland
• PMID: 12053094 (Publication Type: Case Reports, Journal Article)
• Copyright: Copyright 2002 S. Karger AG, Basel

Chemical References

• Indium Radioisotopes
• RNA, Messenger
• Receptors, Somatostatin
• somatostatin receptor 3
• somatostatin receptor type 1
• Pro-Opiomelanocortin
• somatostatin receptor 5
• Adrenocorticotropic Hormone
• Corticotropin-Releasing Hormone
• Hydrocortisone

Topics

• ACTH Syndrome, Ectopic (metabolism)
• Adrenocorticotropic Hormone
• Carcinoma, Medullary (complications, pathology, surgery)
• Corticotropin-Releasing Hormone (genetics)
• Cushing Syndrome (etiology)
• Humans
• Hydrocortisone (blood)
• Indium Radioisotopes
• Lymph Node Excision
• Male
• Middle Aged
• Neoplasm Metastasis
• Pro-Opiomelanocortin (genetics)
• RNA, Messenger (analysis)
• Receptors, Somatostatin (analysis)
• Thyroid Neoplasms (complications, pathology, surgery)
• Thyroidectomy
• Transcription, Genetic