Abstract |
The monoclonal antibodies, 9.2.27 against human melanoma cell lines and WM53 against leukemia cell lines, were conjugated with cyclic anhydride gadolinium-diethylenetriaminepenta-acetic acid (Gd- cDTPAa) and used as tumor-specific contrast agents in magnetic resonance imaging (MRI). The data indicate that Gd-DTPA-9.2.27 in solution decreased the T1 relaxation of water protons at 7.0 Tesla (300 MHz) in direct proportion to the gadolinium concentration, and this effect was greater than in Gd-DTPA solutions. These conjugates show high specificity for melanoma and leukemia cell lines. T1 relaxation time at 7.0 Tesla, measured for the melanoma cell line (MM-138) and leukemia cell line (HL-60) after incubation at 37 degrees C for 4 hr, were significantly decreased (approximately 25%) relative to controls. The gadolinium concentration in cells and washing solutions was measured by inductively coupled plasma atomic emission spectroscopy (ICP-AES). A linear relationship was observed between T1 relaxation rates and gadolinium concentrations obtained by ICP-AES. The ICP-AES results showed no gadolinium uptake in the non-targeted HT-29 colorectal cancer cells.
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Authors | D Shahbazi-Gahrouei, S M Rizvi, M A Williams, B J Allen |
Journal | Australasian physical & engineering sciences in medicine
(Australas Phys Eng Sci Med)
Vol. 25
Issue 1
Pg. 31-8
(Mar 2002)
ISSN: 0158-9938 [Print] Netherlands |
PMID | 12049473
(Publication Type: Comparative Study, Journal Article)
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Chemical References |
- Antibodies, Monoclonal
- Contrast Media
- Radiopharmaceuticals
- Gadolinium DTPA
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Topics |
- Animals
- Antibodies, Monoclonal
(chemistry, pharmacokinetics)
- Contrast Media
(pharmacokinetics)
- Gadolinium DTPA
(chemistry, pharmacokinetics)
- HL-60 Cells
(metabolism)
- HT29 Cells
(metabolism)
- Humans
- Magnetic Resonance Imaging
(methods)
- Melanoma
(metabolism)
- Mice
- Radiopharmaceuticals
(chemistry, pharmacokinetics, therapeutic use)
- Sensitivity and Specificity
- Time Factors
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