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In vitro studies of gadolinium-DTPA conjugated with monoclonal antibodies as cancer-specific magnetic resonance imaging contrast agents.

Abstract
The monoclonal antibodies, 9.2.27 against human melanoma cell lines and WM53 against leukemia cell lines, were conjugated with cyclic anhydride gadolinium-diethylenetriaminepenta-acetic acid (Gd-cDTPAa) and used as tumor-specific contrast agents in magnetic resonance imaging (MRI). The data indicate that Gd-DTPA-9.2.27 in solution decreased the T1 relaxation of water protons at 7.0 Tesla (300 MHz) in direct proportion to the gadolinium concentration, and this effect was greater than in Gd-DTPA solutions. These conjugates show high specificity for melanoma and leukemia cell lines. T1 relaxation time at 7.0 Tesla, measured for the melanoma cell line (MM-138) and leukemia cell line (HL-60) after incubation at 37 degrees C for 4 hr, were significantly decreased (approximately 25%) relative to controls. The gadolinium concentration in cells and washing solutions was measured by inductively coupled plasma atomic emission spectroscopy (ICP-AES). A linear relationship was observed between T1 relaxation rates and gadolinium concentrations obtained by ICP-AES. The ICP-AES results showed no gadolinium uptake in the non-targeted HT-29 colorectal cancer cells.
AuthorsD Shahbazi-Gahrouei, S M Rizvi, M A Williams, B J Allen
JournalAustralasian physical & engineering sciences in medicine (Australas Phys Eng Sci Med) Vol. 25 Issue 1 Pg. 31-8 (Mar 2002) ISSN: 0158-9938 [Print] Netherlands
PMID12049473 (Publication Type: Comparative Study, Journal Article)
Chemical References
  • Antibodies, Monoclonal
  • Contrast Media
  • Radiopharmaceuticals
  • Gadolinium DTPA
Topics
  • Animals
  • Antibodies, Monoclonal (chemistry, pharmacokinetics)
  • Contrast Media (pharmacokinetics)
  • Gadolinium DTPA (chemistry, pharmacokinetics)
  • HL-60 Cells (metabolism)
  • HT29 Cells (metabolism)
  • Humans
  • Magnetic Resonance Imaging (methods)
  • Melanoma (metabolism)
  • Mice
  • Radiopharmaceuticals (chemistry, pharmacokinetics, therapeutic use)
  • Sensitivity and Specificity
  • Time Factors

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