Abstract |
Ubiquitin is a small protein involved in intracellular proteolysis. It is highly conserved throughout eukaryotic phyla and has been detected in such diverse species as yeast, barley, Drosophila and man. A previous study showed that chromatin of rainbow trout testis contains free ubiquitin with a sequence similar to that of other phyla. In the present study, which focused on rainbow trout but included eleven other species, it is shown that fish ubiquitin genetic organisation and expression are similar to those of other phylogenetic groups through the following set of observations: (a) Multiple loci were detected, (b) These loci encode repeats of ubiquitin, (c) Although the DNA sequences are not conserved, the encoded amino acid sequences are fully conserved, (d) The expression of ubiquitin was influenced by cell culture conditions and viral infection.
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Authors | Kazue Okubo, Keisuke Yamano, Qiwei Qin, Kazuhiko Aoyagi, Mitsuru Ototake, Teruyuki Nakanishi, Hideo Fukuda, Johannes M Dijkstra |
Journal | Fish & shellfish immunology
(Fish Shellfish Immunol)
Vol. 12
Issue 4
Pg. 335-51
(Apr 2002)
ISSN: 1050-4648 [Print] England |
PMID | 12049169
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
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Topics |
- Amino Acid Sequence
- Animals
- Base Sequence
- Cells, Cultured
- DNA
(chemistry)
- Gene Expression
- Oncorhynchus mykiss
(genetics, metabolism, virology)
- Phylogeny
- Polymerase Chain Reaction
(veterinary)
- RNA
(chemistry)
- Reverse Transcriptase Polymerase Chain Reaction
(veterinary)
- Sequence Analysis
- Sequence Homology
- Species Specificity
- Ubiquitin
(chemistry, genetics)
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