Antiserum raised against the Epstein-Barr virus BARF0 protein reacts to HLA-DR beta chain.

Abstract

The role that Epstein-Barr virus plays in nasopharyngeal carcinoma and Burkitt's lymphoma has been under intense study for many years. With only a limited set of viral genes being expressed in these tumours it has been difficult to understand how the virus could cause/aid in the generation of the tumours. In 1997 a paper was published by Fries et al. [Fries et al. (1997) Identification of a novel protein encoded by the BamH1 A region of the Epstein-Barr virus. J Virol 71: 2765-2771.] in which a rabbit serum was generated and used to identify protein products (RK-BARF0) encoded from the BamH1 A region of EBV. In this paper we have isolated these proteins from two-dimensional gels and identified them, using mass spectrometry, as components of HLA DR.

• Authors: W Schroder, N Kienzle, G Bushell, T Sculley
• Journal: Archives of virology (Arch Virol) Vol. 147 Issue 4 Pg. 723-9 (Apr 2002) ISSN: 0304-8608 [Print] Austria
• PMID: 12038683 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antibodies, Viral
• BARF1 protein, Human herpesvirus 4
• HLA-DR Antigens
• Immune Sera
• Membrane Proteins
• Viral Proteins

Topics

• Amino Acid Sequence
• Antibodies, Viral (biosynthesis, immunology)
• Cell Line
• Cell Membrane (metabolism)
• Cross Reactions
• Electrophoresis, Gel, Two-Dimensional
• False Positive Reactions
• HLA-DR Antigens (chemistry, immunology)
• Herpesvirus 4, Human (metabolism)
• Humans
• Immune Sera
• Mass Spectrometry
• Membrane Proteins (analysis, immunology)
• Molecular Sequence Data
• Tumor Cells, Cultured
• Viral Proteins (analysis, immunology)