Abstract |
IgE plays a central role in allergic reactions. Some anti-IgE antibodies (HMK-12, 6HD5) inhibit the binding of IgE to the FcepsilonRI of mast cells/basophilic leukemia cells (PT-18, RBL/2H3), but less inhibition is seen with the anti-allotypic JKS-6 and the anti-idiotypic Eb-1. Anti-IgE HMK-12 can detach bound IgE molecules from the FcepsilonRI. When mast cells or basophils were incubated with monoclonal anti-DNP- IgE SPE-7, washed and treated with anti-IgE HMK-12, anti-IgE/ IgE complexes were found in the supernatant. Similar results were obtained with the Fab fragment of HMK-12. Mice injected with anti-DNP- IgE SPE-7 and later with DNP-BSA had the typical systemic anaphylactic shock. However, if they were injected with the anti-IgE antibody (HMK-12) before the challenge, they did not get an anaphylactic shock. In the sera of mice injected with monoclonal IgE SPE-7 and anti-IgE antibody (HMK-12), IgE/ anti-IgE complexes were detected. No passive cutaneous anaphylaxis occurred if the rats were injected with anti-IgE antibodies before the challenge. In summary, anti-IgE antibodies can remove IgE antibodies from the FcepsilonRI; anti-IgE/ IgE complexes can be detected in vitro and in vivo, and anti-IgE antibodies can inhibit IgE-mediated systemic or local anaphylactic reactions.
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Authors | Hiroaki Miyajima, Naohiro Watanabe, Zoltan Ovary, Ko Okumura, Takao Hirano |
Journal | International archives of allergy and immunology
(Int Arch Allergy Immunol)
Vol. 128
Issue 1
Pg. 24-32
(May 2002)
ISSN: 1018-2438 [Print] Switzerland |
PMID | 12037398
(Publication Type: Journal Article)
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Copyright | Copyright 2002 S. Karger AG, Basel |
Chemical References |
- Antibodies, Anti-Idiotypic
- Antibodies, Monoclonal
- Receptors, IgE
- anti-IgE antibodies
- Immunoglobulin E
- Evans Blue
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Topics |
- Anaphylaxis
(immunology, prevention & control)
- Animals
- Antibodies, Anti-Idiotypic
(immunology)
- Antibodies, Monoclonal
(immunology)
- Basophils
(immunology)
- Evans Blue
(chemistry)
- Female
- Flow Cytometry
- Hypersensitivity, Immediate
(immunology)
- Immunoglobulin E
(immunology)
- Leukemia, Basophilic, Acute
- Mast Cells
(immunology)
- Mice
- Mice, Inbred BALB C
- Passive Cutaneous Anaphylaxis
(immunology)
- Rats
- Rats, Sprague-Dawley
- Receptors, IgE
(immunology)
- Skin
(immunology)
- Tumor Cells, Cultured
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