Abstract |
Combination chemotherapy with gemcitabine (Gem), doxorubicin (Dox), and paclitaxel (Pac) ( GAT) has been considered attractive as first-line treatment in metastatic breast cancer. We compared the potential of various schedules of GAT to induce apoptosis on MDA-MB-231, MCF7, and T47D human breast cancer cell lines. The extent of apoptotic induction was analyzed by flow cytometry with 7-aminoactinomycin D (7AAD) staining. Differences between various schedules in terms of apoptotic induction were statistically significant (P < 0.05). The most effective apoptotic induction regimen was achieved by the sequence: Dox for 16 h followed by Pac + Gem. Schedules employing a 16-h interval between drug administrations induced higher levels of apoptosis in human breast cancer cell lines compared with schedules using a 4-h interval. The therapeutic efficacy of the experimental results shown in this paper has been clinically corroborated in a phase II trial in metastatic breast cancer patients.
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Authors | Maria J Serrano, Pedro Sánchez-Rovira, Ignacio Algarra, Ana Jaén, Ana Lozano, José J Gaforio |
Journal | Japanese journal of cancer research : Gann
(Jpn J Cancer Res)
Vol. 93
Issue 5
Pg. 559-66
(May 2002)
ISSN: 0910-5050 [Print] Japan |
PMID | 12036452
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antibiotics, Antineoplastic
- Deoxycytidine
- Dactinomycin
- Doxorubicin
- Paclitaxel
- Gemcitabine
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Topics |
- Antibiotics, Antineoplastic
(pharmacology)
- Antineoplastic Combined Chemotherapy Protocols
(therapeutic use)
- Apoptosis
- Breast Neoplasms
(metabolism, pathology)
- Cell Division
- Cell Survival
- Clinical Trials as Topic
- Dactinomycin
(pharmacology)
- Deoxycytidine
(administration & dosage, analogs & derivatives)
- Dose-Response Relationship, Drug
- Doxorubicin
(administration & dosage)
- Flow Cytometry
- Humans
- Paclitaxel
(administration & dosage)
- Time Factors
- Tumor Cells, Cultured
- Gemcitabine
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