G6PD hillbrow: a new variant of glucose-6-phosphate dehydrogenase associated with drug-induced haemolytic anaemia.

Abstract

A new genetic variant of the red cell enzyme glucose-6-phosphate dehydrogenase is described. It was observed in a patient presenting with severe haemolytic anaemia and renal failure following ingestion of an overdose of Beserol (paracetamol and chlormezanone). The enzyme in the red cell had 12% of the activity of a normal B+ control, but only slightly lower activity in the kidney compared with a normal control. The red cell enzyme showed normal electrophoretic mobility and thermostability, a biphasic pH optimum curve, higher than normal utilization of the substrate analogues 2-deoxy-glucose-6-phosphate and deamino-NADP, and lower than normal Michaelis constants for both substrates, glucose-6-phosphate and NADP. The enzyme was strongly inhibited in vitro by high concentrations of paracetamol and chlormezanone. The extent of inhibition was similar to that for the enzyme from a normal B+ individual.

• Authors: E Cayanis, E D Gomperts, D Balinsky, P Disler, A Myers
• Journal: British journal of haematology (Br J Haematol) Vol. 30 Issue 3 Pg. 343-50 (Jul 1975) ISSN: 0007-1048 [Print] England
• PMID: 1201217 (Publication Type: Journal Article)

Chemical References

• Drug Combinations
• acetaminophen, caffeine, carisoprodol, diclofenac drug combination
• Diclofenac
• Carisoprodol
• Acetaminophen
• Caffeine
• Glucosephosphate Dehydrogenase
• Chlormezanone

Topics

• Acetaminophen (pharmacology)
• Adult
• Anemia, Hemolytic (chemically induced)
• Caffeine
• Carisoprodol
• Chlormezanone (pharmacology)
• Diclofenac
• Drug Combinations
• Erythrocyte Aging (drug effects)
• Erythrocytes (enzymology)
• Genetic Variation
• Glucosephosphate Dehydrogenase (antagonists & inhibitors, blood, isolation & purification)
• Humans
• Male