Groups of metabolically normal (controls) and
alloxan-diabetic adult female rabbits were fed semi-synthetic diets containing 40 cal %
palm-kernel oil (PKO) or
sunflower-seed oil (SSO) for 54 weeks. In contrast to control rabbits fed PKO-diet, the
alloxan-diabetic rabbits on this diet, developed no or only a negligible degree of
atherosclerosis, although the serum levels of all
lipid classes had increased in the diabetic rabbits above that of the controls during almost the whole experimental period. The diabetic rabbits and the controls fed SSO-diet were both free from any significant atherosclerotic involvement in spite of the fact that the SSO-diet appeared unable to suppress the very high levels of the various serum
lipid classes induced by the diabetic state. On both diets, the diabetic rabbits showed a significantly higher
cholesteryl linoleate/
oleate ratio than the controls, which was caused by an increase in the
cholesteryl linoleate level in the diabetics. No serious aorta
atherosclerosis was found in rabbits with a
cholesteryl linoleate/
oleate ratio higher than 0.6, although no correlation was found between the
atherosclerosis indices and these ratios at values lower than 0.6. Rabbits with
cholesteryl linoleate/
oleate ratios below 0.6 seemed to run a greater risk of developing
atherosclerosis. It is suggested that
insulin might be required for
atherogenesis in addition to
hyperlipemia and
hypercholesterolemia.