Abstract |
The profile of cytokines induced by soluble leishmania antigen (SLA) and the Leishmania homologue of the mammalian receptor for activated C kinase (LACK), a candidate vaccine against leishmaniasis, and the cellular source of the cytokines produced in response to these antigens were analyzed in patients infected with Leishmania guyanensis. Gamma interferon (IFN-gamma) and interleukin-10 (IL-10) were produced in response to LACK. Although LACK-specific CD4(+) cells producing IFN-gamma were isolated only during the early phase of infection (less than 30 days following the onset of infection), cells producing IL-10 in response to LACK were detected in all patients. CD4(+) T cells producing IFN-gamma and IL-13 were produced in response to SLA in all patients. SLA- and LACK-specific T cells are effector memory cells, as they are CD45RA(-) CCR7(-) CD4(+) T cells. CD4(+) T cells producing IFN-gamma are CD62L(-), and CD4(+) T cells producing IL-10 are CD62L(+), indicating that these cells have different tissue-homing capacities. These findings show that SLA and LACK induce both type 1 (IFN-gamma) and type 2 (IL-10 or IL-13) cell responses.
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Authors | Eliane Bourreau, Ghislaine Prévot, Jacques Gardon, Roger Pradinaud, Hitoshi Hasagewa, Geneviève Milon, Pascal Launois |
Journal | Infection and immunity
(Infect Immun)
Vol. 70
Issue 6
Pg. 3122-9
(Jun 2002)
ISSN: 0019-9567 [Print] United States |
PMID | 12011006
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antigens, Protozoan
- Biomarkers
- CCR7 protein, human
- Interleukin-13
- Peptides
- Protozoan Proteins
- Receptors, CCR7
- Receptors, Chemokine
- L-Selectin
- Interleukin-10
- LACK antigen, Leishmania
- Interferon-gamma
- Leukocyte Common Antigens
- Protein Tyrosine Phosphatase, Non-Receptor Type 1
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Topics |
- Amino Acid Sequence
- Animals
- Antigens, Protozoan
(immunology)
- Biomarkers
- CD4-Positive T-Lymphocytes
(immunology)
- Cells, Cultured
- Humans
- Interferon-gamma
(biosynthesis)
- Interleukin-10
(metabolism)
- Interleukin-13
(biosynthesis)
- L-Selectin
- Leishmania guyanensis
(immunology)
- Leishmaniasis, Mucocutaneous
(blood, immunology, pathology)
- Leukocyte Common Antigens
- Leukocytes, Mononuclear
(cytology, immunology)
- Molecular Sequence Data
- Peptides
(immunology)
- Protein Tyrosine Phosphatase, Non-Receptor Type 1
- Protozoan Proteins
(immunology)
- Receptors, CCR7
- Receptors, Chemokine
- Time Factors
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