Low-density lipoprotein reconstituted by pyropheophorbide cholesteryl oleate as target-specific photosensitizer.

Abstract

To target tumors overexpressing low-density lipoprotein receptors (LDLr), a pyropheophorbide cholesterol oleate conjugate was synthesized and successfully reconstituted into the low-density lipoprotein (LDL) lipid core. Laser scanning confocal microscopy studies demonstrated that this photosensitizer-reconstituted LDL can be internalized via LDLr by human hepatoblastoma G(2) (HepG(2)) tumor cells.

• Authors: Gang Zheng, Hui Li, Min Zhang, Sissel Lund-Katz, Britton Chance, Jerry D Glickson
• Journal: Bioconjugate chemistry (Bioconjug Chem) 2002 May-Jun Vol. 13 Issue 3 Pg. 392-6 ISSN: 1043-1802 [Print] United States
• PMID: 12009925 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Cholesterol Esters
• Lipoproteins, LDL
• Photosensitizing Agents
• Receptors, LDL
• Chlorophyll
• pyropheophorbide a
• cholesteryl oleate

Topics

• Binding, Competitive
• Chlorophyll (analogs & derivatives, chemistry, metabolism, pharmacology)
• Cholesterol Esters (chemistry, metabolism, pharmacology)
• Endosomes (metabolism)
• Humans
• Lipoproteins, LDL (metabolism)
• Lysosomes (metabolism)
• Microscopy, Confocal
• Microscopy, Fluorescence
• Photosensitizing Agents (chemistry, pharmacology)
• Receptors, LDL (chemistry, metabolism)
• Tumor Cells, Cultured (drug effects, metabolism)