Immunisation of dairy cattle with recombinant Streptococcus uberis GapC or a chimeric CAMP antigen confers protection against heterologous bacterial challenge.

Abstract	The gapC genes, encoding the cell surface-associated GapC proteins of S. uberis and S. agalactiae, have been cloned and sequenced. To identify potential vaccine candidates against S. uberis-induced bovine mastitis, lactating dairy cows were vaccinated with either (6 x His)GapC of S. uberis or S. dysgalactiae, or with a chimeric CAMP-factor antigen, CAMP-3. For 7 days following heterologous challenge with S. uberis, milk somatic cell counts were determined to assess differences in the severity of mastitis between vaccinates and an unvaccinated control group. Vaccination with S. uberis (6 x His)GapC or CAMP-3 resulted in a significant reduction in inflammation on several days post-challenge, most significantly for the former antigen. Inflammation was not reduced in S. dysgalactiae (6 x His)GapC vaccinates, suggesting that it does not confer cross-species protection.
Authors	Michael C Fontaine, Jose Perez-Casal, Xin Ming Song, Jim Shelford, Philip J Willson, Andrew A Potter
Journal	Vaccine (Vaccine) Vol. 20 Issue 17-18 Pg. 2278-86 (May 22 2002) ISSN: 0264-410X [Print] Netherlands
PMID	12009283 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References	Antigens, Bacterial Bacterial Proteins CAMP protein, Streptococcus DNA, Bacterial GapC protein, Streptococcus Hemolysin Proteins Recombinant Proteins Streptococcal Vaccines Vaccines, Synthetic
Topics	Animals Antigens, Bacterial (genetics, immunology) Bacterial Proteins (genetics, immunology) Base Sequence Cattle Cloning, Molecular DNA, Bacterial Female Gene Expression Genes, Bacterial Hemolysin Proteins Mastitis, Bovine (microbiology, prevention & control) Molecular Sequence Data Recombinant Proteins (genetics, immunology) Recombination, Genetic Streptococcal Vaccines (genetics, immunology) Streptococcus (genetics, immunology) Vaccination Vaccines, Synthetic (genetics, immunology)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.

Realize the full power of the drug-disease research graph!