Immunoelectron microscopic analysis of lysosomal deposits in alpha-N-acetylgalactosaminidase deficiency with angiokeratoma corporis diffusum.

Abstract

Alpha-N-acetylgalactosaminidase (alpha-NAGA) deficiency with angiokeratoma corporis diffusum (AKCD) is one of the lysosomal storage diseases. GalNAc(alpha))1-O-Ser/Thr (Tn) is theoretically deposited in lysosomes, but substances with attached galactose and neuraminic (sialic) acid (T and sialosyl Tn, respectively) are excreted in patients' urine. In this study, in two Japanese cases we analyzed the material accumulated in lysosomes using immunoelectron microscopy with mouse antibodies to Tn, sialosyl Tn and T (Thomsen-Friedenreich) antigens in order to find out what substance(s) is really deposited in lysosomes. We found that only GalNAc(alpha)1-O-Ser/Thr (Tn) was actually accumulated in vacuolated lysosomes of vascular endothelial cells, eccrine sweat gland cells, fibroblasts and pericytes. Galactosylation and sialylation of Tn appears to occur in cells other than those in the skin. The results suggest that this disease is caused by a single enzyme deficiency.

• Authors: Akira Kanda, Shinichiro Tsuyama, Fusayoshi Murata, Kazuo Kodama, Yoshio Hirabayashi, Tamotsu Kanzaki
• Journal: Journal of dermatological science (J Dermatol Sci) Vol. 29 Issue 1 Pg. 42-8 (May 2002) ISSN: 0923-1811 [Print] Netherlands
• PMID: 12007720 (Publication Type: Journal Article)

Chemical References

• Antibodies, Monoclonal
• Antigens, Tumor-Associated, Carbohydrate
• Tn antigen
• sialosyl-Tn antigen
• Thomsen-Friedenreich antigen
• Hexosaminidases
• NAGA protein, human
• alpha-N-Acetylgalactosaminidase

Topics

• Animals
• Antibodies, Monoclonal
• Antigens, Tumor-Associated, Carbohydrate (analysis)
• Fabry Disease (enzymology)
• Female
• Hexosaminidases (deficiency)
• Humans
• Japan
• Lysosomes (ultrastructure)
• Male
• Mice
• Microscopy, Immunoelectron
• Middle Aged
• alpha-N-Acetylgalactosaminidase