Abstract |
In strokes, myocardial infarctions, severe sustained hemorrhagic shock, and donor organs, inadequate blood supply results in lack of oxygen to the tissue ( ischemia). If ischemia is sustained, reperfusion with the needed oxygen can result in tissue injury (ischemia-reperfusion injury) due to formation of reactive oxygen species. We are studying an oxygen-carrying solution with anitoxidant activity formed by cross-linking hemoglobin, superoxide dismutase, and catalase to form PolyHb-SOD-CAT. The present report studies its effect on the blood-brain barrier and cerebral edema when used in a transient global brain ischemia-reperfusion rat model. We compare this solution to sham-control, oxygenated saline, stroma-free hemoglobin (SF-Hb), polymerized hemoglobin (PolyHb), and a mixture of SF-Hb, SOD, and CAT in free solution. The results show that the cross-linked PolyHb-SOD-CAT solution, unlike the other solutions, can supply oxygen to ischemic tissues without causing reperfusion injury in the transient global brain ischemia-reperfusion model.
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Authors | D Douglas Powanda, Thomas M S Chang |
Journal | Artificial cells, blood substitutes, and immobilization biotechnology
(Artif Cells Blood Substit Immobil Biotechnol)
Vol. 30
Issue 1
Pg. 23-37
(Jan 2002)
ISSN: 1073-1199 [Print] England |
PMID | 12000224
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Blood Substitutes
- Coloring Agents
- Hemoglobins
- Oxyhemoglobins
- Water
- Evans Blue
- Catalase
- Superoxide Dismutase
- polyhemoglobin-superoxide dismutase-catalase
- Oxygen
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Topics |
- Animals
- Blood Substitutes
(chemistry, pharmacology)
- Blood-Brain Barrier
(physiology)
- Brain Edema
(prevention & control)
- Brain Ischemia
(drug therapy, metabolism)
- Catalase
(chemistry, metabolism, pharmacology)
- Coloring Agents
(pharmacokinetics)
- Evans Blue
(pharmacokinetics)
- Hemoglobins
(chemistry, pharmacology)
- Male
- Molecular Weight
- Oxygen
(metabolism)
- Oxyhemoglobins
(metabolism)
- Rats
- Rats, Sprague-Dawley
- Reperfusion Injury
(drug therapy, metabolism)
- Superoxide Dismutase
(chemistry, metabolism, pharmacology)
- Water
(metabolism)
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