Effect of Traumacel P on the growth of human dermal fibroblasts in vitro.

Abstract

OBJECTIVE:

It has been postulated that Traumacel P, a haemostatic powder, might assist the wound-healing process. This in vitro study investigated the effect of the powder on human dermal fibroblasts.

METHOD:

Experiments using fibroblasts from a 50-year-old donor were carried out using suspensions of Traumacel P ranging from 0.1 to 10.0 mg/ml. Dulbecco's Modified Eagle Medium with 10% or 0.4% foetal calf serum (FCS) and 5.5 mM or 25 mM glucose was used. The powder was either placed in contact with the cells or separated from them by a porous membrane. The response of the fibroblasts was assessed using the MTT assay.

RESULTS:

Concentrations of 0.5 mg/ml and 1.0 mg/ml stimulated the metabolic activity of the fibroblasts in both high and low glucose medium with 10% FCS, regardless of whether the powder was in contact with or separated from the cells. The greatest stimulation, to 174% of the controls, was produced by 0.5 mg/ml Traumacel P in low glucose/10% FCS with the powder in contact with the cells (p < 0.0001). Traumacel concentrations ranging from 0.5 to 5.0 mg/ml in 0.4% FCS significantly stimulated the metabolic activity of fibroblasts in low glucose medium, but not in the high glucose medium.

CONCLUSION:

These studies indicate that direct stimulation of fibroblast proliferation may be one of the ways in which Traumacel P aids the healing of ulcers observed in the clinic.

• Authors: M A Hughes, Y Yang, George W Cherry
• Journal: Journal of wound care (J Wound Care) Vol. 11 Issue 4 Pg. 149-54 (Apr 2002) ISSN: 0969-0700 [Print] England
• PMID: 11998596 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Hemostatics
• Uronic Acids
• traumacel

Topics

• Cells, Cultured
• Female
• Fibroblasts (drug effects, metabolism)
• Hemostatics (pharmacology, therapeutic use)
• Humans
• Middle Aged
• Uronic Acids (pharmacology, therapeutic use)
• Wound Healing (drug effects)