Abstract |
Norwalk Virus and Norwalk-like viruses (NLVs) are reportedly responsible for 2.5-4.0% of nonbacterial acute gastroenteritis ( NBAG) worldwide. To help clarify the impact of NLVs on NBAG in Indonesia, stool specimens from 102 patients, 74 with NBAG and 28 with BAG, were screened for the presence of NLVs, using a reverse transcription-polymerase chain reaction (RT-PCR) assay. The specimens were subtyped using prototype-specific oligonucleotide probes and were sequenced and compared with published NLV sequences. Of the 102 specimens examined, 31 (30%) were found to be positive for NLVs. Type-specific probe analysis of the RT-PCR products indicated that 31 isolates hybridized to UK1 (Taunton agent) and UK3/4 (Hawaii agent/Snow Mountain agent) prototype strains. The results of this study indicate that prototype strains of NV or NLVs co-circulate in Indonesia and contribute to the overall level of acute gastroenteritis throughout the region.
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Authors | D S Subekti, P Tjaniadi, M Lesmana, C Simanjuntak, S Komalarini, H Digdowirogo, B Setiawan, A L Corwin, J R Campbell, K R Porter, B A Oyofo |
Journal | Journal of medical virology
(J Med Virol)
Vol. 67
Issue 2
Pg. 253-8
(Jun 2002)
ISSN: 0146-6615 [Print] United States |
PMID | 11992587
(Publication Type: Journal Article, Research Support, U.S. Gov't, Non-P.H.S.)
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Copyright | Copyright 2002 Wiley-Liss, Inc. |
Topics |
- Acute Disease
- Base Sequence
- Caliciviridae Infections
(virology)
- Child
- Child, Preschool
- Feces
(virology)
- Gastroenteritis
(virology)
- Humans
- Indonesia
- Infant
- Infant, Newborn
- Molecular Sequence Data
- Norovirus
(classification, genetics)
- Reverse Transcriptase Polymerase Chain Reaction
- Sequence Analysis, DNA
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