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Characterization of Norwalk-like virus associated with gastroenteritis in Indonesia.

Abstract
Norwalk Virus and Norwalk-like viruses (NLVs) are reportedly responsible for 2.5-4.0% of nonbacterial acute gastroenteritis (NBAG) worldwide. To help clarify the impact of NLVs on NBAG in Indonesia, stool specimens from 102 patients, 74 with NBAG and 28 with BAG, were screened for the presence of NLVs, using a reverse transcription-polymerase chain reaction (RT-PCR) assay. The specimens were subtyped using prototype-specific oligonucleotide probes and were sequenced and compared with published NLV sequences. Of the 102 specimens examined, 31 (30%) were found to be positive for NLVs. Type-specific probe analysis of the RT-PCR products indicated that 31 isolates hybridized to UK1 (Taunton agent) and UK3/4 (Hawaii agent/Snow Mountain agent) prototype strains. The results of this study indicate that prototype strains of NV or NLVs co-circulate in Indonesia and contribute to the overall level of acute gastroenteritis throughout the region.
AuthorsD S Subekti, P Tjaniadi, M Lesmana, C Simanjuntak, S Komalarini, H Digdowirogo, B Setiawan, A L Corwin, J R Campbell, K R Porter, B A Oyofo
JournalJournal of medical virology (J Med Virol) Vol. 67 Issue 2 Pg. 253-8 (Jun 2002) ISSN: 0146-6615 [Print] United States
PMID11992587 (Publication Type: Journal Article, Research Support, U.S. Gov't, Non-P.H.S.)
CopyrightCopyright 2002 Wiley-Liss, Inc.
Topics
  • Acute Disease
  • Base Sequence
  • Caliciviridae Infections (virology)
  • Child
  • Child, Preschool
  • Feces (virology)
  • Gastroenteritis (virology)
  • Humans
  • Indonesia
  • Infant
  • Infant, Newborn
  • Molecular Sequence Data
  • Norovirus (classification, genetics)
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sequence Analysis, DNA

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