[Determination of medullasin levels for the diagnosis of multiple sclerosis].

Abstract

Medullasin levels in granulocytes of patients with neurological diseases and healthy volunteers were determined by the enzyme immunoassay using mouse monoclonal antibodies against human medullasin and o-phenylenediamine-H2O2 as the detection system of the enzyme activity. One hundred twenty-one out of 159 patients with multiple sclerosis (76.1%) showed positive results (above means of normals + 2SD) in this test, while only 16.9% (24/142) of patients with non-inflammatory neurological diseases had positive results. This enzyme immunoassay method for medullasin is considered to be an useful paraclinical test for the diagnosis of multiple sclerosis.

• Authors: Yosuke Aoki, Takahiko Saida, Imaharu Nakano, Toyokazu Saito, Kunihiko Ikeguchi, Takao Urabe, Etsuko Nishiguchi, Hideaki Suzuki, Kiyoshi Takahashi, Yoshikuni Mizuno
• Journal: Rinsho byori. The Japanese journal of clinical pathology (Rinsho Byori) Vol. 50 Issue 3 Pg. 308-12 (Mar 2002) ISSN: 0047-1860 [Print] Japan
• PMID: 11985062 (Publication Type: English Abstract, Journal Article)

Chemical References

• Serine Endopeptidases
• medullasin

Topics

• Adolescent
• Adult
• Child
• Female
• Granulocytes (enzymology)
• Humans
• Immunoenzyme Techniques
• Male
• Middle Aged
• Multiple Sclerosis (diagnosis)
• Serine Endopeptidases (blood)