Abstract |
The human kallikreins are a large multigene family of closely related serine-type proteases. In this regard, they are similar to the multigene kallikrein families characterized in mice and rats. There is a much more extensive body of knowledge regarding the function of mouse and rat kallikreins in comparison with the human kallikreins. Human kallikrein 6 has been proposed as the homologue to rat myelencephalon-specific protease, an arginine-specific degradative-type protease abundantly expressed in the central nervous system and implicated in demyelinating disease. We present the x-ray crystal structure of mature, active recombinant human kallikrein 6 at 1.75-A resolution. This high resolution model provides the first three-dimensional view of one of the human kallikreins and one of only a few structures of serine proteases predominantly expressed in the central nervous system. Enzymatic data are presented that support the identification of human kallikrein 6 as the functional homologue of rat myelencephalon-specific protease and are corroborated by a molecular phylogenetic analysis. Furthermore, the x-ray data provide support for the characterization of human kallikrein 6 as a degradative protease with structural features more similar to trypsin than the regulatory kallikreins.
|
Authors | Matthew J Bernett, Sachiko I Blaber, Isobel A Scarisbrick, Pushparani Dhanarajan, Steven M Thompson, Michael Blaber |
Journal | The Journal of biological chemistry
(J Biol Chem)
Vol. 277
Issue 27
Pg. 24562-70
(Jul 05 2002)
ISSN: 0021-9258 [Print] United States |
PMID | 11983703
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
|
Chemical References |
- KLK6 protein, human
- Kallikreins
- Prss18 protein, mouse
- Trypsin
|
Topics |
- Amino Acid Sequence
- Animals
- Central Nervous System
(enzymology)
- Consensus Sequence
- Humans
- Kallikreins
(chemistry, genetics, metabolism)
- Mice
- Models, Molecular
- Molecular Sequence Data
- Protein Conformation
- Rats
- Sequence Alignment
- Sequence Homology, Amino Acid
- Trypsin
(chemistry, metabolism)
|