Abstract | BACKGROUND/AIMS: METHODS:
Carrageenan was intraperitoneally injected once into mice and phenotypic and functional characterizations were conducted in various immune organs. RESULTS: Natural killer (NK) cells were prominently activated in the liver, lungs, and spleen. A time-kinetic study showed sequential activation of NK and natural killer T (NKT) cells in the liver on days 3-10 after the injection. In parallel with the activation of NK and NKT cells in number, NK and NKT cytotoxicities were augmented. At this time, liver injury was induced, accompanied by massive hepatic necrosis and the elevation of transaminases. The in vivo elimination of NK cells reduced the liver injury induced by carrageenan. Direct binding of carrageenan onto NK cells was also demonstrated. Such a binding then induced a subsequent production of IFN gamma. Perforin molecules of NK cells were responsible for this liver injury. CONCLUSIONS: These results suggest that not only phagocytic cells but also primitive lymphocyte (mainly NK cells) subsets might be important targets for the acute toxicity of carrageenan.
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Authors | Tetsuya Abe, Hiroki Kawamura, Shotetsu Kawabe, Hisami Watanabe, Fumitake Gejyo, Toru Abo |
Journal | Journal of hepatology
(J Hepatol)
Vol. 36
Issue 5
Pg. 614-23
(May 2002)
ISSN: 0168-8278 [Print] Netherlands |
PMID | 11983444
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Interferon-gamma
- Carrageenan
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Topics |
- Animals
- Carrageenan
(pharmacology)
- Cell Division
(immunology)
- Interferon-gamma
(biosynthesis)
- Killer Cells, Natural
(cytology, drug effects, immunology)
- Kinetics
- Liver
(cytology, immunology)
- Liver Diseases
(immunology)
- Lung
(cytology, immunology)
- Lymphocyte Activation
(drug effects)
- Mice
- Mice, Inbred BALB C
- Mice, Inbred C57BL
- Mice, Knockout
- Spleen
(cytology, immunology)
- T-Lymphocytes, Cytotoxic
(cytology, drug effects, immunology)
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