Grape seed proanthocyanidins improved cardiac recovery during reperfusion after ischemia in isolated rat hearts.

Abstract

BACKGROUND:

Increasing evidence shows that red wine consumption has cardioprotective effects. These effects have been attributed to the polyphenolic compounds in grapes.

OBJECTIVE:

We studied the effects of red grape seed proanthocyanidins on the recovery of postischemic function in isolated rat hearts.

DESIGN:

Two groups of rats were fed different doses of proanthocyanidin-rich extract for 3 wk and another group was untreated and served as controls. The animals were then anesthetized and the hearts were isolated and subjected to 30 min of ischemia followed by 2 h of reperfusion. Coronary effluents were collected during the third minute of reperfusion for measurement of oxygen free radicals by using electron spin resonance spectroscopy.

RESULTS:

In rats treated with 50 and 100 mg grape seed proanthocyanidins/kg, the incidence of reperfusion-induced ventricular fibrillation was reduced from its control value of 92% to 42% and 25%, respectively (P < 0.05 for both). The incidence of ventricular tachycardia showed the same pattern. In rats treated with 100 mg proanthocyanidins/kg, the recovery of coronary flow, aortic flow, and developed pressure after 60 min of reperfusion was improved by 32% +/- 8%, 98% +/- 8%, and 37% +/- 3%, respectively (P < 0.05 for all) compared with untreated control rats. Electron spin resonance studies indicated that proanthocyanidins significantly inhibited the formation of oxygen free radicals. In rats treated with 100 mg proanthocyanidins/kg, free radical intensity was reduced by 75% +/- 7% (P < 0.05) compared with the control rats.

CONCLUSION:

Grape seed proanthocyanidins have cardioprotective effects against reperfusion-induced injury via their ability to reduce or remove, directly or indirectly, free radicals in myocardium that is reperfused after ischemia.

• Authors: Tunde Pataki, Istvan Bak, Peter Kovacs, Debasis Bagchi, Dipak K Das, Arpad Tosaki
• Journal: The American journal of clinical nutrition (Am J Clin Nutr) Vol. 75 Issue 5 Pg. 894-9 (May 2002) ISSN: 0002-9165 [Print] United States
• PMID: 11976164 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Anthocyanins
• Antioxidants
• Cardiotonic Agents
• Proanthocyanidins
• Reactive Oxygen Species
• proanthocyanidin

Topics

• Animals
• Anthocyanins (isolation & purification, pharmacology)
• Antioxidants (isolation & purification, pharmacology)
• Aorta (physiopathology)
• Cardiotonic Agents (isolation & purification, pharmacology)
• Coronary Circulation (drug effects)
• Heart (drug effects, physiopathology)
• In Vitro Techniques
• Myocardial Ischemia (physiopathology)
• Myocardial Reperfusion Injury (physiopathology)
• Proanthocyanidins
• Rats
• Rats, Sprague-Dawley
• Reactive Oxygen Species (antagonists & inhibitors)
• Recovery of Function
• Regional Blood Flow (drug effects)
• Seeds (chemistry)
• Vitis (chemistry)