Analysis of Helicobacter pylori and nonsteroidal anti-inflammatory drug-induced gastric epithelial injury.

Abstract

BACKGROUND:

Helicobacter pylori and nonsteroidal anti-inflammatory drugs (NSAIDs) are important factors in gastric mucosal injury. However, the relationship between H. pylori and NSAID-related gastroduodenal mucosal injury has not been clarified.

AIM:

To determine the role of H. pylori in NSAID-induced gastric mucosal injury and to examine the effects of H. pylori, indomethacin and sofalcone on gastric epithelial cells in culture, as a useful model to study gastric mucosal injury. In addition, we studied the effect of sofalcone, a gastric mucosal protection agent, on H. pylori and NSAID-induced gastric mucosal injury.

METHODS:

Cytotoxic and noncytotoxic strains of H. pylori were used, each with an inoculum of 10(7) cfu/mL. The effect on the growth of RGM-1 cells (a rat gastric epithelial cell line) was studied by MTT assay, and levels of prostaglandin E2 in culture supernatants were measured by EIA.

RESULTS:

Both cytotoxic and noncytotoxic strains of H. pylori tended to induce cell injury in RGM-1 cells at 48 h after inoculation. Indomethacin alone induced gastric epithelial injury in a dose-dependent manner, but did not augment cell injury induced by H. pylori. In addition, sofalcone (10(-5) mol/L) showed a suppressive effect on indomethacin-induced gastric epithelial injury.

CONCLUSION:

These findings indicate that indomethacin induces gastric mucosal injury regardless of H. pylori infection, and suggests that sofalcone may be a useful drug in the treatment of NSAID-induced mucosal injury.

• Authors: M Igarashi, A Takagi, X Jiang, K Hasumi, S Watanabe, R Deguchi, T Miwa
• Journal: Alimentary pharmacology & therapeutics (Aliment Pharmacol Ther) Vol. 16 Suppl 2 Pg. 235-9 (Apr 2002) ISSN: 0269-2813 [Print] England
• PMID: 11966547 (Publication Type: Journal Article)

Chemical References

• Anti-Inflammatory Agents, Non-Steroidal
• Indomethacin

Topics

• Animals
• Anti-Inflammatory Agents, Non-Steroidal (toxicity)
• Cell Line
• Cell Survival (drug effects, physiology)
• Epithelial Cells (drug effects, pathology)
• Gastric Mucosa (drug effects, pathology)
• Helicobacter Infections (microbiology, physiopathology)
• Helicobacter pylori (isolation & purification)
• Indomethacin (toxicity)
• Rats