Abstract |
The procedure for isolating soluble melanoprotein from human malignant melanoma was described. Electron microscopy confirmed the absence of any contaminating organized particles. The melanoprotein was brown hygroscopic substance with 8.8% of melanin, easily soluble in water. The solution revealed general absorption with a shoulder at 280 nm. Chemical composition was typical for a melanoprotein complex with characteristic high sulphur content (2.1%) and abundance in zinc (210 mug Zn/g dry sample). Sedimentation and electrophoretic properties indicated relative homogeneity. Similar substance could also be isolated from Harding-Passey mouse melanoma. Such soluble melanoproteins have been needed for their broad exploitation in tumor immunology.
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Authors | J Borovanský, P Hach, J Duchon |
Journal | Neoplasma
(Neoplasma)
Vol. 22
Issue 5
Pg. 525-30
( 1975)
ISSN: 0028-2685 [Print] Slovakia |
PMID | 1196435
(Publication Type: Journal Article)
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Chemical References |
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Topics |
- Female
- Humans
- Melanoma
(analysis)
- Neoplasm Proteins
(analysis, isolation & purification)
- Skin Neoplasms
(analysis)
- Solubility
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