Abstract | OBJECTIVE: To study the effects of soybean trypsin inhibitor (TI) on glycine uptake, glutathione (GSH) levels and morphological changes of intestine in rotavirus (RV) infected infant mice. METHODS: A total of 144 infant mice (7/8 days old) were divided in 3 groups (i.e. control, RV and RV + inhibitor). Infant mice were orally inoculated with the EB strain of RV and Trypsin protease inhibitor (TI) and 8 animals each were sacrificed on days 0,1,3,5,7 and 10 post infection (p.i). Glycine uptake (in vitro), GSH levels and histological changes were assessed in the jejunum, ileum and colon. RESULTS:
Glycine uptake and GSH levels were significantly reduced on days 3 and 5 p.i in jejunum and ileum of RV inoculated animals, compared to the controls. Glycine uptake and GSH levels were maintained as in controls in the RV + TI inoculated animals on days 3 and 5 p.i in jejunum and colon but not in ileum where lesser values were recorded. Histology showed vacuolar degeneration in ileum towards the apical portion whereas normal morphology was observed in jejunum, similar to controls. No histological changes were observed in colon in any of the groups. Electron microscopic study confirmed the viral infection. CONCLUSION:
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Authors | R Katyal, S V Rana, S Ojha, K Vaiphei, V Singh, K Singh |
Journal | Tropical gastroenterology : official journal of the Digestive Diseases Foundation
(Trop Gastroenterol)
2001 Oct-Dec
Vol. 22
Issue 4
Pg. 207-10
ISSN: 0250-636X [Print] India |
PMID | 11963327
(Publication Type: Journal Article)
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Chemical References |
- Trypsin Inhibitors
- Trypsin Inhibitor, Kunitz Soybean
- Glutathione
- Glycine
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Topics |
- Animals
- Animals, Newborn
- Body Weight
(drug effects)
- Diarrhea
(etiology, prevention & control)
- Glutathione
(metabolism)
- Glycine
(metabolism)
- Intestinal Mucosa
(pathology, virology)
- Mice
- Mice, Inbred BALB C
- Models, Animal
- Rotavirus Infections
(complications, drug therapy, metabolism)
- Trypsin Inhibitor, Kunitz Soybean
(pharmacology, therapeutic use)
- Trypsin Inhibitors
(pharmacology, therapeutic use)
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