Relationship between expression of matrix metalloproteinase-2 and matrix metalloproteinase-9 and invasion ability of cervical cancer cells.

Abstract

Constitutive overexpression of matrix metalloproteinases (MMPs) is frequently observed in malignant tumors. MMPs are a family of zinc endopeptidases consisting of at least 20 different members. In particular, MMP-2 and MMP-9 are reported to be closely associated with invasion and metastasis in several cancers. We investigated whether expression of MMP-2 and MMP-9 is associated with invasion ability of seven cervical cancer cells by administration of o-phenanthroline as MMP inhibitor. In two cell lines, Siha and Caski, MMP-2 mRNA and protein were expressed at high levels. After treatment with o-phenanthroline, the rate of invasion in these two cell lines was significantly decreased. In contrast, in the other two cell lines, HT-3 and Caski, high levels of MMP-9 mRNA and protein were expressed but there was no decrease in the rate of invasion in these cells after treatment with o-phenanthroline. The data suggest that expression level of MMP-2 mRNA may regulate with invasion ability of cervical cancer.

• Authors: Yasuhito Kato, Tsuyoshi Yamashita, Mutsuo Ishikawa
• Journal: Oncology reports (Oncol Rep) 2002 May-Jun Vol. 9 Issue 3 Pg. 565-9 ISSN: 1021-335X [Print] Greece
• PMID: 11956628 (Publication Type: Journal Article)

Chemical References

• Phenanthrolines
• Protease Inhibitors
• RNA, Messenger
• Matrix Metalloproteinase 2
• Matrix Metalloproteinase 9
• 1,10-phenanthroline

Topics

• Female
• Humans
• Matrix Metalloproteinase 2 (biosynthesis)
• Matrix Metalloproteinase 9 (biosynthesis)
• Neoplasm Invasiveness
• Phenanthrolines (pharmacology)
• Protease Inhibitors (pharmacology)
• RNA, Messenger (metabolism)
• Reverse Transcriptase Polymerase Chain Reaction
• Tumor Cells, Cultured
• Uterine Cervical Neoplasms (enzymology, pathology)