Abstract |
Laminin-1, a heterotrimer of alpha 1, beta 1, and gamma 1 chains specific to basement membrane, promotes cell adhesion and migration, proteinase secretion and metastases of tumour cells. Several active sites on the alpha 1 chain have been found to promote B16-F10 melanoma lung colonisation and here we have determined whether additional tumour promoting sites exist on the beta 1 and gamma 1 chains. Recently, we have identified novel cell adhesive peptides derived from laminin beta 1 and gamma 1 chains by systematic screening of synthetic peptides. Nine beta 1 peptides and seven gamma 1 peptides active for cell adhesion were tested for their effects on experimental pulmonary metastases of B16-F10 mouse melanoma cells in vivo. The most active adhesive peptide derived from the gamma 1 chain globular domain, C-16 (KAFDITYVRLKF), significantly enhanced pulmonary metastases of B16-F10 cells, whereas no other peptides showed enhancement. C-16 also stimulated migration of B16-F10 cells in the Boyden chamber assay in vitro. Furthermore, C-16 significantly induced the production of MMP-9 from B16-F10 cells. These results suggest that this specific laminin gamma 1 chain peptide has a metastasis-promoting activity and might be a new molecular target of anti- cancer treatment.
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Authors | Y Kuratomi, M Nomizu, K Tanaka, M L Ponce, S Komiyama, H K Kleinman, Y Yamada |
Journal | British journal of cancer
(Br J Cancer)
Vol. 86
Issue 7
Pg. 1169-73
(Apr 08 2002)
ISSN: 0007-0920 [Print] England |
PMID | 11953867
(Publication Type: Journal Article)
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Chemical References |
- Laminin
- Peptide Fragments
- laminin gamma 1
- Matrix Metalloproteinase 9
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Topics |
- Animals
- Cell Adhesion
- Cell Movement
(drug effects)
- Disease Models, Animal
- Laminin
(pharmacology)
- Lung Neoplasms
(secondary, veterinary)
- Matrix Metalloproteinase 9
(metabolism)
- Melanoma
(pathology, veterinary)
- Mice
- Neoplasm Metastasis
(physiopathology)
- Peptide Fragments
(pharmacology)
- Tumor Cells, Cultured
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