Abstract | BACKGROUND: METHODS:
IGFBP-3 was re-expressed by stable transfection of human IGFBP-3 cDNA in a model of human prostate cancer, M12, a malignant subline in which IGFBP-3 levels are undetectable in comparison to the parent epithelial cell, P69. Effect of IGFBP-3 re-expression (M12-BP-3) on growth kinetics, morphology, propensity to apoptosis, and in vivo tumor formation were studied. RESULTS: M12-BP-3 cells secreted IGFBP-3 and growth arrested at a cell density that was threefold lower than control cells and this was associated with marked alteration in cell morphology. Control cells when grown in conditioned media secreted by M12-BP-3 also showed altered morphology compared to when cultured in IGFBP-3-immunodepleted conditioned media. The M12-BP-3 clones showed altered mitochondrial membrane potential, increased PARP cleavage, increase in sub-G1 peak, decreased levels of neuron specific enolase, and decreased tumor formation in athymic, nude mice. CONCLUSIONS: These data suggest that IGFBP-3 induces early apoptosis and has potential tumor suppressive effect in prostate cancer. Prostate 51: 141-152, 2002.
|
Authors | Gayathri R Devi, Cynthia C Sprenger, Stephen R Plymate, Ron G Rosenfeld |
Journal | The Prostate
(Prostate)
Vol. 51
Issue 2
Pg. 141-52
(May 01 2002)
ISSN: 0270-4137 [Print] United States |
PMID | 11948969
(Publication Type: Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, U.S. Gov't, P.H.S.)
|
Copyright | Copyright 2002 Wiley-Liss, Inc. |
Chemical References |
- Culture Media
- DNA, Complementary
- DNA, Neoplasm
- Insulin-Like Growth Factor Binding Protein 3
|
Topics |
- Apoptosis
(drug effects)
- Blotting, Western
- Cell Division
(drug effects)
- Cell Transformation, Neoplastic
- Culture Media
- DNA, Complementary
(genetics)
- DNA, Neoplasm
(genetics)
- Electrophoresis, Polyacrylamide Gel
- Epithelial Cells
(physiology)
- Gene Expression Regulation, Neoplastic
- Humans
- Immunohistochemistry
- Insulin-Like Growth Factor Binding Protein 3
(biosynthesis, pharmacology)
- Male
- Prostatic Neoplasms
(pathology)
- Tumor Cells, Cultured
|