Antiangiogenic and antitumor activity of IDN 5390, a new taxane derivative.

Abstract

PURPOSE:

We previously reported that paclitaxel, a microtubule-stabilizing drug, inhibited angiogenesis, mainly by inhibiting endothelial cell motility (D. Belotti et al., Clin. Cancer Res., 2: 1843-1849, 1996). The aim of this study was to select a taxane with little cytotoxicity but with antimotility and hence antiangiogenic activity.

EXPERIMENTAL DESIGN:

Different taxanes, seco derivatives, and 14-beta-hydroxy-10-deacetyl baccatin III derivatives were tested for their effects on the proliferation and motility of human umbilical vein endothelial cells. The antiangiogenic and antineoplastic activities of the compound selected from this screening were further investigated in experimental models in vitro and in vivo.

RESULTS:

From the screening of different taxanes, we selected IDN 5390, a seco derivative that showed potent antimotility activity and less cytotoxicity than paclitaxel. In comparable experimental conditions, IDN 5390 inhibited endothelial cell migration without affecting proliferation. This compound dose-dependently inhibited the capacity of human umbilical vein endothelial cells plated on Matrigel to organize into a network of cords. In vivo, IDN 5390 significantly inhibited fibroblast growth factor-2-induced angiogenesis in Matrigel implants. Daily treatment with IDN 5390 in mice bearing established lung micrometastases from the B16BL6 murine melanoma caused a reduction in the size of metastases. Finally, IDN 5390 slowed the s.c. growth of the paclitaxel-resistant human ovarian carcinoma, 1A9/PTX22, xenografted in nude mice.

CONCLUSIONS:

The seco derivative IDN 5390 might represent the prototype of a new class of taxane derivatives with antiangiogenic properties.

• Authors: Giulia Taraboletti, Gianluca Micheletti, Monica Rieppi, Maura Poli, Michele Turatto, Cosmo Rossi, Patrizia Borsotti, Paola Roccabianca, Eugenio Scanziani, Maria Ines Nicoletti, Ezio Bombardelli, Paolo Morazzoni, Antonella Riva, Raffaella Giavazzi
• Journal: Clinical cancer research : an official journal of the American Association for Cancer Research (Clin Cancer Res) Vol. 8 Issue 4 Pg. 1182-8 (Apr 2002) ISSN: 1078-0432 [Print] United States
• PMID: 11948131 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Angiogenesis Inhibitors
• Antineoplastic Agents
• Bridged-Ring Compounds
• IDN 5390
• Taxoids
• Tubulin
• Fibroblast Growth Factor 2
• taxane
• Paclitaxel

Topics

• Angiogenesis Inhibitors (pharmacology)
• Animals
• Antineoplastic Agents (pharmacology)
• Bridged-Ring Compounds (chemistry, pharmacology)
• Cell Division (drug effects)
• Cell Line
• Cell Movement (drug effects)
• Dose-Response Relationship, Drug
• Drug Resistance, Neoplasm
• Endothelium, Vascular (cytology, drug effects, metabolism)
• Female
• Fibroblast Growth Factor 2 (pharmacology)
• Humans
• Lung Neoplasms (prevention & control, secondary)
• Melanoma, Experimental (pathology, prevention & control)
• Mice
• Mice, Inbred C57BL
• Mice, Nude
• Neoplasm Transplantation
• Neovascularization, Physiologic (drug effects)
• Ovarian Neoplasms (pathology, prevention & control)
• Paclitaxel (analogs & derivatives, pharmacology)
• Taxoids
• Time Factors
• Tubulin (drug effects, metabolism)
• Tumor Cells, Cultured
• Xenograft Model Antitumor Assays