Association of the phosphatidylinositol signal pathway with prolonged myocardial ischemia.

Abstract

OBJECTIVE:

To study the changes in activity of phosphatidylinositol 4 kinase (PI 4 kinase), phosphatidylinositol 4 phosphate 5 kinase (PIP 5 kinase) and protein kinase C (PKC) during myocardial ischemia and elucidate the relationship between phosphatidylinositol signal pathways and prolonged myocardial ischemia.

METHODS:

In vivo an ischemic rat model was used. Activity of PI 4 kinase, PIP 5 kinase and PKC were measured at different times in postischemic heart cells using isotope analysis.

RESULTS:

The activity of PI kinase, PIP kinase and PKC in the myocardium increased to peak at 1 hour postischemia, with activities 6.1, 3.0 and 4.0 fold over control levels, respectively. Their activities declined to normal levels with time.

CONCLUSION:

The phosphatidylinositol signal pathway is involved in prolonged myocardial ischemia, but its mechanism needs further study.

• Authors: Xiuyun Ding, Jiachang Yue, Shiwen Wang, Xue Gao, Xianfeng Li
• Journal: Chinese medical journal (Chin Med J (Engl)) Vol. 115 Issue 3 Pg. 367-70 (Mar 2002) ISSN: 0366-6999 [Print] China
• PMID: 11940366 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Phosphotransferases (Alcohol Group Acceptor)
• 1-Phosphatidylinositol 4-Kinase
• 1-phosphatidylinositol-4-phosphate 5-kinase
• Protein Kinase C

Topics

• 1-Phosphatidylinositol 4-Kinase (metabolism)
• Animals
• Male
• Myocardial Ischemia (enzymology)
• Phosphotransferases (Alcohol Group Acceptor) (metabolism)
• Protein Kinase C (metabolism)
• Random Allocation
• Rats
• Rats, Wistar
• Signal Transduction