[Study on mechanism of Shenle and Fragmin in preventing and treating glomerulosclerosis in rats].

Abstract

OBJECTIVE:

To study the effects of Shenle and Fragmin on the degradation of extracellular matrix metalloproteinase-9 (MMP-9) and tissue inhibitors of metalloproteinase-1 (TIMP-1) in the remnant kidneys of 5/6 nephrectomized rats treated with Shenle and Fragmin.

METHODS:

The model of focal and segmental glomerulosclerosis was made by 5/6 nephrectomy in Wistar rats. After being treated with Shenle (4 g.kg-1.d-1) or Fragmin (1000 IU.kg-1.d-1) for 20 weeks, the blood pressure, proteinuria, serum creatinine, lipids, renal pathological change of remnant kidney of the model animals were examined, and the mRNA expression of MMP-9, TIMP-1 and type IV collagen in the remnant kidney were also detected.

RESULTS:

Shenle and Fragmin could significantly reduce the blood pressure, proteinuria, serum creatinine and lipids, alleviate the pathologic change of kidney tissue, decrease the type IV collagen, MMP-9 deposition. Shenle and Fragmin down-regulated the mRNA expression of MMP-9 by 30.0% and 28.5% and TIMP-1 expression by 59.3% and 55.0% in the remnant kidney of the 5/6 nephrectomized rats respectively.

CONCLUSION:

Shenle and Fragmin might ameliorate glomerulosclerosis through modulating MMP-9/TIMP-1 gene expression, alleviate the abnormal metabolism and accumulation, promote extracellular matrix degradation in glomeruli remodeling, it might be the important mechanism of Shenle and Fragmin in treating glomerulosclerosis.

• Authors: W Wang, X Chen, Y Ye
• Journal: Zhongguo Zhong xi yi jie he za zhi Zhongguo Zhongxiyi jiehe zazhi = Chinese journal of integrated traditional and Western medicine (Zhongguo Zhong Xi Yi Jie He Za Zhi) Vol. 20 Issue 12 Pg. 923-7 (Dec 2000) ISSN: 1003-5370 [Print] China
• PMID: 11938866 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Drugs, Chinese Herbal
• Hirudins
• RNA, Messenger
• Tissue Inhibitor of Metalloproteinase-1
• Collagen
• Matrix Metalloproteinase 9
• Dalteparin

Topics

• Animals
• Collagen (biosynthesis, genetics)
• Dalteparin (therapeutic use)
• Drugs, Chinese Herbal (therapeutic use)
• Female
• Glomerulosclerosis, Focal Segmental (drug therapy, prevention & control)
• Hirudins
• Matrix Metalloproteinase 9 (biosynthesis, genetics)
• Nephrectomy
• RNA, Messenger (genetics)
• Random Allocation
• Rats
• Rats, Wistar
• Tissue Inhibitor of Metalloproteinase-1 (biosynthesis, genetics)