Abstract |
Two pimarane diterpenes structurally related to sphaeropsidins were isolated from the liquid culture of Sphaeropsis sapinea f. sp. cupressi, a plant pathogenic fungus causing a form of canker disease of Italian cypress (Cupressus sempervirens L.). The two metabolites, characterised by spectroscopic methods, were named sphaeropsidins D (0.40 mg l(-1)) and E (0.16 mg l(-1)). The same fungus produced sphaeropsidins A, B and C, sphaeropsidone and episphaeropsidone, which proved to be phytotoxic to cypress, and chlorosphaeropsidone and epichlorosphaeropsidone showing no phytotoxicity. Sphaeropsidin D assayed at 0.1 mg ml(-1) on severed cypress twigs caused leaf browning and necrosis on Cupressus macrocarpa, but no symptoms were observed on C. sempervirens and C. arizonica. Symptoms appeared in a period of time (6 days after toxin-treatment) shorter than that for sphaeropsidin A. Sphaeropsidin E assayed at 0.2 mg ml(-1) did not produce any symptom on the same cypress species tested with sphaeropsidin D.
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Authors | Antonio Evidente, Lorenzo Sparapano, Giovanni Bruno, Andrea Motta |
Journal | Phytochemistry
(Phytochemistry)
Vol. 59
Issue 8
Pg. 817-23
(Apr 2002)
ISSN: 0031-9422 [Print] England |
PMID | 11937160
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Diterpenes
- sphaeropsidin D
- sphaeropsidin E
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Topics |
- Biological Assay
- Culture Techniques
- Cupressus
(drug effects, physiology)
- Diterpenes
(chemistry, isolation & purification, pharmacology)
- Mitosporic Fungi
(chemistry)
- Plant Diseases
(microbiology)
- Toxicity Tests
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