Abstract | BACKGROUND: METHODS: Primary cultures of fibroblasts were obtained from Dupuytren's affected fascia and carpal tunnel affected fascia as a control. Collagen lattices were prepared and populated with the fibroblasts. The fibroblast-populated collagen lattices (FPCL) were then measured for contraction every 24 h for 5 days. Supernatant was obtained from the culture medium following completion of the FPCL portion of the experiment and used for a TGF(beta2) immunoassay. RESULTS: Dupuytren's affected fibroblasts contracted the FPCLs significantly more than carpal tunnel control fibroblasts. Treating the fibroblasts with tamoxifen caused a decreased contraction rate in both Dupuytren's affected fibroblasts and carpal tunnel controls. There was increased TGF(beta2) expression in the Dupuytren's affected fascia group compared to the carpal tunnel control group. Tamoxifen decreased TGF(beta2) expression in Dupuytren's affected fascia group but not in the carpal tunnel control group. CONCLUSION:
TGF(beta) appears to be the key cytokine in the fibrogenic nature of Dupuytren's disease. Tamoxifen treatment has been demonstrated to decrease the function of fibroblasts derived from Dupuytren's affected fascia and downregulated TGF(beta2) production in these same fibroblasts. These data suggest a method to manipulate and control Dupuytren's contracture in the clinical setting.
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Authors | M Ann Kuhn, X Wang, Wyatt G Payne, Francis Ko, Martin C Robson |
Journal | The Journal of surgical research
(J Surg Res)
Vol. 103
Issue 2
Pg. 146-52
(Apr 2002)
ISSN: 0022-4804 [Print] United States |
PMID | 11922728
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Estrogen Antagonists
- TGFB2 protein, human
- Transforming Growth Factor beta
- Transforming Growth Factor beta2
- Tamoxifen
- Collagen
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Topics |
- Carpal Tunnel Syndrome
(pathology)
- Cell Culture Techniques
- Collagen
- Dupuytren Contracture
(drug therapy, pathology, physiopathology)
- Estrogen Antagonists
(pharmacology)
- Fascia
(chemistry, drug effects, pathology)
- Fibroblasts
(drug effects, physiology)
- Humans
- Immunoassay
- Tamoxifen
(pharmacology)
- Transforming Growth Factor beta
(analysis)
- Transforming Growth Factor beta2
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