Phage display particles expressing tumor-specific antigens induce preventive and therapeutic anti-tumor immunity in murine p815 model.

Abstract

The efficacy of phage display particles expressing tumor antigen P1A35-43 in inducing protective and therapeutic anti-tumor immune responses were studied. A protective immune response against a lethal progressive P815 mastocytoma tumor cell challenge was established after subcutaneous injection of phage display particles. Furthermore, the vaccine suppressed growth of pre-existing tumors. Immunization with the hybrid phage particles elicited P1A(35-43) specific CTL responses and a Th1-dominated immune response with phage particle-specific secretion of IFN-gamma but not IL-4. Our results indicate that phage display particles might be a useful vaccine form for tumor-associated antigen epitopes in tumor immunotherapy.

• Authors: Yuzhang Wu, Ying Wan, Jiang Bian, Jianping Zhao, ZhengCai Jia, Liyun Zhou, Wei Zhou, Yang Tan
• Journal: International journal of cancer (Int J Cancer) Vol. 98 Issue 5 Pg. 748-53 (Apr 10 2002) ISSN: 0020-7136 [Print] United States
• PMID: 11920646 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright 2002 Wiley-Liss, Inc.

Chemical References

• DNA Primers
• Peptide Fragments
• Peptide Library
• Chromium
• Interleukin-4
• Interferon-gamma

Topics

• Animals
• Base Sequence
• Chromium (metabolism)
• DNA Primers
• Immunotherapy
• Interferon-gamma (metabolism)
• Interleukin-4 (metabolism)
• Male
• Mice
• Mice, Inbred DBA
• Molecular Sequence Data
• Neoplasms, Experimental (immunology, mortality, therapy)
• Peptide Fragments (immunology)
• Peptide Library
• Plasmids
• Survival Rate
• Th1 Cells (immunology)