Cyclotheonamide E4 and E5, new potent tryptase inhibitors from an Ircinia species of sponge.

Abstract

Tryptase is a protease released from mast cells and is believed to contribute to the inflammatory process in allergic diseases including asthma. In the course of screening to find tryptase inhibitors, we isolated two new tryptase inhibitors, cyclotheonamide E4 (3) and E5 (4), from a marine sponge of the genus Ircinia. The structures of these molecules were determined by interpretation of 1H and 13C NMR spectra, and they were shown to be closely related to the previously reported cyclotheonamides E (1), E2, and E3 (2). These molecules contain two unusual amino acids, vinylogous tyrosine and alpha-ketohomoarginine, which are involved in strong activities against serine proteases. Cyclotheonamide E4 showed potent inhibitory activity against human tryptase (IC50 5.1 nM). Therefore, cyclotheonamide E4 may be useful as a therapeutic agent in the treatment of allergic diseases including asthma.

• Authors: Yasunobu Murakami, Masao Takei, Kazutoshi Shindo, Chie Kitazume, Junichi Tanaka, Tatsuo Higa, Hiromi Fukamachi
• Journal: Journal of natural products (J Nat Prod) Vol. 65 Issue 3 Pg. 259-61 (Mar 2002) ISSN: 0163-3864 [Print] United States
• PMID: 11908961 (Publication Type: Journal Article)

Chemical References

• Peptides, Cyclic
• Serine Proteinase Inhibitors
• Tpsb2 protein, mouse
• cyclotheonamide E
• cyclotheonamide E2
• cyclotheonamide E3
• cyclotheonamide E4
• cyclotheonamide E5
• Serine Endopeptidases
• Tpsab1 protein, mouse
• Tryptases

Topics

• Animals
• Cells, Cultured (drug effects)
• Humans
• Japan
• Mast Cells (drug effects)
• Mice
• Mice, Inbred CBA
• Molecular Structure
• Nuclear Magnetic Resonance, Biomolecular
• Peptides, Cyclic (chemistry, isolation & purification, pharmacology)
• Porifera (chemistry)
• Serine Endopeptidases (metabolism)
• Serine Proteinase Inhibitors (chemistry, isolation & purification, pharmacology)
• Tryptases