Noladin ether, a putative novel endocannabinoid: inactivation mechanisms and a sensitive method for its quantification in rat tissues.

Abstract

The occurrence of the novel proposed endocannabinoid, noladin ether (2-arachidonyl glyceryl ether, 2-AGE) in various rat organs and brain regions, and its inactivation by intact C6 glioma cells, were studied. 2-AGE was measured by isotope dilution liquid chromatography-atmospheric pressure chemical ionization-mass spectrometry, with a detection limit of 100 fmol. A compound with the same mass and chromatographic/chemical properties as 2-AGE was found in whole brain, with the highest amounts in the thalamus and hippocampus. Synthetic [(3)H]2-AGE was inactivated by intact rat C6 glioma cells by a time- and temperature-dependent process consisting of cellular uptake and partial incorporation into phospholipids. Further data suggested that 2-AGE is taken up by cells via the anandamide/2-arachidonoyl glycerol (2-AG) membrane transporter(s), and biosynthesized in a different way as compared to 2-AG.

• Authors: Filomena Fezza, Tiziana Bisogno, Alberto Minassi, Giovanni Appendino, Raphael Mechoulam, Vincenzo Di Marzo
• Journal: FEBS letters (FEBS Lett) Vol. 513 Issue 2-3 Pg. 294-8 (Feb 27 2002) ISSN: 0014-5793 [Print] England
• PMID: 11904167 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Cannabinoid Receptor Modulators
• Cannabinoids
• Endocannabinoids
• Glycerides
• noladin ether

Topics

• Analysis of Variance
• Animals
• Brain (metabolism)
• Cannabinoid Receptor Modulators
• Cannabinoids (analysis, antagonists & inhibitors)
• Endocannabinoids
• Glycerides (analysis, antagonists & inhibitors)
• Male
• Rats
• Rats, Sprague-Dawley