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Investigation of the role of Endo180/urokinase-type plasminogen activator receptor-associated protein as a collagenase 3 (matrix metalloproteinase 13) receptor.

Abstract
Procollagenase 3 can be activated by interaction with and cleavage by the cell-associated membrane type 1 metalloproteinase (MT1 MMP; MMP 14). It has also been shown to bind to a specific receptor, and is subsequently internalized via the low-density lipoprotein-related receptor by osteoblast cell lines. The receptor was identified as a recycling glycoprotein of the macrophage mannose receptor family, Endo180. In order to ascertain whether there is a relationship between Endo180 binding and procollagenase 3 activation, we have compared procollagenase 3 activation by an HT1080 fibrosarcoma cell line overexpressing MT1 MMP, without and with overexpression of Endo180. No difference in procollagenase 3 activation was observed, and neither was the enzyme bound to the cells or internalized. In contrast, the osteoblast cell lines, MG63 and UMR-106, both bound and internalized procollagenase 3. However, immunolocalization studies showed that the Endo180 abundantly expressed by these cells did not co-localize with the procollagenase 3. In further biochemical studies we confirmed that procollagenase 3 did not bind to Endo180, using both ligand- blotting and immunoprecipitation techniques. We conclude that Endo180 is unlikely to be a receptor for collagenase 3 in relation to either its activation or cell binding and internalization, and that other interaction partners must be sought.
AuthorsLouise Bailey, Dirk Wienke, Matthew Howard, Vera Knäuper, Clare M Isacke, Gillian Murphy
JournalThe Biochemical journal (Biochem J) Vol. 363 Issue Pt 1 Pg. 67-72 (Apr 01 2002) ISSN: 0264-6021 [Print] England
PMID11903048 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • DNA, Complementary
  • Endo180
  • Ligands
  • PLAUR protein, human
  • RNA, Messenger
  • Receptors, Cell Surface
  • Receptors, Mitogen
  • Receptors, Urokinase Plasminogen Activator
  • Collagenases
  • MMP13 protein, human
  • Matrix Metalloproteinase 13
Topics
  • Blotting, Western
  • Cell Line
  • Collagenases (metabolism)
  • DNA, Complementary (metabolism)
  • Humans
  • Ligands
  • Matrix Metalloproteinase 13
  • Microscopy, Fluorescence
  • Osteoblasts (metabolism)
  • Precipitin Tests
  • Protein Binding
  • RNA, Messenger (metabolism)
  • Receptors, Cell Surface (chemistry, metabolism)
  • Receptors, Mitogen (chemistry, metabolism)
  • Receptors, Urokinase Plasminogen Activator
  • Time Factors
  • Transfection
  • Tumor Cells, Cultured

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