Abstract |
Procollagenase 3 can be activated by interaction with and cleavage by the cell-associated membrane type 1 metalloproteinase (MT1 MMP; MMP 14). It has also been shown to bind to a specific receptor, and is subsequently internalized via the low-density lipoprotein-related receptor by osteoblast cell lines. The receptor was identified as a recycling glycoprotein of the macrophage mannose receptor family, Endo180. In order to ascertain whether there is a relationship between Endo180 binding and procollagenase 3 activation, we have compared procollagenase 3 activation by an HT1080 fibrosarcoma cell line overexpressing MT1 MMP, without and with overexpression of Endo180. No difference in procollagenase 3 activation was observed, and neither was the enzyme bound to the cells or internalized. In contrast, the osteoblast cell lines, MG63 and UMR-106, both bound and internalized procollagenase 3. However, immunolocalization studies showed that the Endo180 abundantly expressed by these cells did not co-localize with the procollagenase 3. In further biochemical studies we confirmed that procollagenase 3 did not bind to Endo180, using both ligand- blotting and immunoprecipitation techniques. We conclude that Endo180 is unlikely to be a receptor for collagenase 3 in relation to either its activation or cell binding and internalization, and that other interaction partners must be sought.
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Authors | Louise Bailey, Dirk Wienke, Matthew Howard, Vera Knäuper, Clare M Isacke, Gillian Murphy |
Journal | The Biochemical journal
(Biochem J)
Vol. 363
Issue Pt 1
Pg. 67-72
(Apr 01 2002)
ISSN: 0264-6021 [Print] England |
PMID | 11903048
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- DNA, Complementary
- Endo180
- Ligands
- PLAUR protein, human
- RNA, Messenger
- Receptors, Cell Surface
- Receptors, Mitogen
- Receptors, Urokinase Plasminogen Activator
- Collagenases
- MMP13 protein, human
- Matrix Metalloproteinase 13
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Topics |
- Blotting, Western
- Cell Line
- Collagenases
(metabolism)
- DNA, Complementary
(metabolism)
- Humans
- Ligands
- Matrix Metalloproteinase 13
- Microscopy, Fluorescence
- Osteoblasts
(metabolism)
- Precipitin Tests
- Protein Binding
- RNA, Messenger
(metabolism)
- Receptors, Cell Surface
(chemistry, metabolism)
- Receptors, Mitogen
(chemistry, metabolism)
- Receptors, Urokinase Plasminogen Activator
- Time Factors
- Transfection
- Tumor Cells, Cultured
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