Cellular location of proteins related to iron absorption and transport.

Abstract	K562 erythroleukemia cells and IEC6 rat cells were examined using confocal microscopy and antibodies raised against DMT-1 (Nramp-2, DCT-1), transferrin receptor (CD71), beta(3) integrin (CD61), mobilferrin (calreticulin), and Hephaestin. The cellular location of each of these proteins was identified by immunofluorescence in both saponin-permeabilized and non-permeabilized cells. Fluorescent reactivity was observed on or near the cell surface of each of these proteins, suggesting that they might participate in surface membrane transport of iron. Fluorescence was observed in the region of the cytoplasm with each antibody to include beta(3) integrin and transferrin receptor. It was pronounced in cells incubated with mobilferrin, Hephaestin, and DMT-1 antibodies. Speckled nuclear fluorescence was observed in cells incubated with anti-DMT-1. While these observations are descriptive, they demonstrate that there are significant concentrations of DMT-1, mobilferrin, and Hephaestin in the cytoplasmic region of cells. This suggests that there may be intracellular roles for these proteins in addition to their serving to transit iron across the cell surface membrane.
Authors	Marcia J Simovich, Marcel E Conrad, Jay N Umbreit, Elizabeth G Moore, Lucille N Hainsworth, Hilary K Smith
Journal	American journal of hematology (Am J Hematol) Vol. 69 Issue 3 Pg. 164-70 (Mar 2002) ISSN: 0361-8609 [Print] United States
PMID	11891802 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)
Chemical References	Antigens, CD Carrier Proteins Cation Transport Proteins HEPH protein, human Heph, protein, rat Integrin beta3 Iron-Binding Proteins Membrane Proteins Platelet Membrane Glycoproteins Proteins Receptors, Transferrin mobilferrin solute carrier family 11- (proton-coupled divalent metal ion transporters), member 2 Iron
Topics	Absorption Animals Antigens, CD (metabolism) Carrier Proteins (metabolism) Cation Transport Proteins Cell Compartmentation Cell Membrane (metabolism) Cell Nucleus (metabolism) Cytoplasm (metabolism) Humans Integrin beta3 Ion Transport Iron (pharmacokinetics) Iron-Binding Proteins Membrane Proteins (metabolism) Microscopy, Fluorescence Platelet Membrane Glycoproteins (metabolism) Protein Transport Proteins (metabolism) Rats Receptors, Transferrin (metabolism) Tumor Cells, Cultured

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