[Inhibition of monocytes adhesion to the intima of arterial wall by local expression of antisense monocyte chemotactic protein-1].

Abstract

OBJECTIVE:

To study the mechanism of monocyte recruitment in atherogenesis and to clarify the effect of monocyte chemotactic protein-1 (MCP-1) in this process.

METHODS:

Femoral arteries isolated from the rabbits which had been fed with a high cholesterol diet and locally perfused with MM-LDL within the artery beforehand, were used as the models. Antisense MCP-1cDNA was transferred into the arterial wall by injecting recombinant LNCX-anti-MCP-1/liposomal complex in the femoral sheath and the periarterial tissue.

RESULTS:

Expression of antisense MCP-1 mediated by recombinant LNCX plasmid/lipsomal complex gene transfer enabled to inhibit MCP-1 gene expression and adhesion of monocyte to the intima.

CONCLUSION:

MCP-1 plays an important role on the recruitment of monocytes in the arterial wall, which provides a potential clue in developing a gene therapy project for the prevention and treatment of atherogenesis.

• Authors: Q Wu, H Qiao, Z Wang, H Zhang, P Liu, M Xu, G Ren, S Zhao, M She
• Journal: Zhonghua bing li xue za zhi = Chinese journal of pathology (Zhonghua Bing Li Xue Za Zhi) Vol. 29 Issue 2 Pg. 107-10 (Apr 2000) ISSN: 0529-5807 [Print] China
• PMID: 11866901 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Chemokine CCL2
• DNA, Antisense
• Lipoproteins, LDL
• RNA, Messenger
• oxidized low density lipoprotein

Topics

• Animals
• Arteriosclerosis (etiology, pathology)
• Cell Adhesion
• Chemokine CCL2 (biosynthesis, genetics)
• DNA, Antisense (biosynthesis, genetics)
• Femoral Artery (ultrastructure)
• Lipoproteins, LDL (pharmacology)
• Male
• Monocytes (physiology)
• Muscle, Smooth, Vascular (metabolism)
• Myocytes, Smooth Muscle (metabolism)
• RNA, Messenger (genetics)
• Rabbits
• Tunica Intima (ultrastructure)