Abstract | OBJECTIVE: METHODS: The study population included 11 patients with H. pylori infection, 12 with ZES, 17 with both H. pylori infection and ZES, and three control subjects with neither. Using a competitive polymerase chain reaction for interleukin (IL)-1beta, IL-6, IL-8, and tumor necrosis factor-alpha, the polymerase chain reaction products in gastric biopsies were quantitated by capillary electrophoresis and laser-induced fluorescence. RESULTS: The levels of IL-1beta, IL-6, IL-8, and tumor necrosis factor-a mRNA in gastric tissue of patients with H. pylori infection only and ZES only exceeded the levels in the control gastric tissue (p < 0.05 to p < 0.005). Unexpectedly, the number of molecules of IL-1beta and IL-8 mRNA in gastric tissue from ZES patients exceeded the levels in gastric tissue from patients with H. pylori only (p < 0.05). The local levels of cytokine mRNA in patients with both diseases exceeded the levels in patients with H. pylori only (IL-6, p < 0.05; IL-8, p < 0.05) and ZES only (IL-6, p < 0.05; tumor necrosis factor-a, p < 0.05). CONCLUSIONS:
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Authors | Paul R Harris, H Christian Weber, C Mel Wilcox, Robert T Jensen, Phillip D Smith |
Journal | The American journal of gastroenterology
(Am J Gastroenterol)
Vol. 97
Issue 2
Pg. 312-8
(Feb 2002)
ISSN: 0002-9270 [Print] United States |
PMID | 11866267
(Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S., Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- Biomarkers
- Cytokines
- RNA, Messenger
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Topics |
- Adult
- Aged
- Analysis of Variance
- Biomarkers
(analysis)
- Case-Control Studies
- Cytokines
(analysis, genetics)
- Female
- Gastric Mucosa
(pathology)
- Gene Expression
- Helicobacter Infections
(genetics, pathology)
- Helicobacter pylori
(isolation & purification)
- Humans
- Male
- Middle Aged
- Probability
- RNA, Messenger
(analysis)
- Reference Values
- Reverse Transcriptase Polymerase Chain Reaction
- Sensitivity and Specificity
- Zollinger-Ellison Syndrome
(genetics, pathology)
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