Abstract | OBJECTIVES: STUDY DESIGN: Seventy-five experimental mice with severe combined immunodeficiency disease were inoculated orally with Candida albicans by swab 3 times a week for a 4-week period. On treatment day, mice were cultured for baseline fungal growth and received a topical oral cavity administration of 0.05 mL MB solution at one of the following concentrations: 250, 275, 300, 350, 400, 450, or 500 microgram/mL. After 10 minutes the mice were recultured and underwent light activation with 664 nm of diode laser light with a cylindrical diffuser. After photodynamic therapy the mice were cultured again for colony-forming units per milliliter and then killed, their tissue harvested for histopathology. RESULTS AND CONCLUSIONS: The results indicate an MB dose-dependent effect. Concentrations from 250 to 400 microgram/mL reduced fungal growth but did not eliminate Candida albicans. MB concentrations of 450 and 500 microgram/mL totally eradicated Candida albicans from the oral cavity, resulting in reductions from 2.5 log(10) and 2.74 log(10) to 0, respectively. These results suggest that MB-mediated photodynamic therapy can potentially be used to treat oral candidiasis in immunodeficient patients.
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Authors | M C Teichert, J W Jones, M N Usacheva, M A Biel |
Journal | Oral surgery, oral medicine, oral pathology, oral radiology, and endodontics
(Oral Surg Oral Med Oral Pathol Oral Radiol Endod)
Vol. 93
Issue 2
Pg. 155-60
(Feb 2002)
ISSN: 1079-2104 [Print] United States |
PMID | 11862203
(Publication Type: Evaluation Study, Journal Article, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- Antifungal Agents
- Azoles
- Photosensitizing Agents
- Methylene Blue
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Topics |
- Animals
- Antifungal Agents
(pharmacology)
- Azoles
(pharmacology)
- Candida albicans
(drug effects)
- Candidiasis, Oral
(drug therapy, microbiology)
- Colony Count, Microbial
- Disease Models, Animal
- Dose-Response Relationship, Drug
- Drug Resistance, Fungal
- Methylene Blue
(administration & dosage, therapeutic use)
- Mice
- Mice, Nude
- Mice, SCID
- Photochemotherapy
(methods)
- Photosensitizing Agents
(administration & dosage, therapeutic use)
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