Abstract | BACKGROUND: MATERIALS AND METHODS: The cytotoxicity parameters were determined following protocols established by the National Cancer Institute (NCI) using the SRB assay. In the topoisomerase assay, the supercoiled DNA produces open circle forms that are stabilised in the presence of DNA topoisomerase poisons and can be detected after a denaturation step by proteinase K-SDS. RESULTS: CONCLUSION: These results could justify the traditional use of the studied annonaceus species and topoisomerase-mediated DNA damage might be a possible mechanism by which five of these compounds exert their cytotoxicity.
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Authors | M López-Lázaro, C Martín-Cordero, A Bermejo, D Cortes, M J Ayuso |
Journal | Anticancer research
(Anticancer Res)
2001 Sep-Oct
Vol. 21
Issue 5
Pg. 3493-7
ISSN: 0250-7005 [Print] Greece |
PMID | 11848514
(Publication Type: Journal Article)
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Chemical References |
- Antineoplastic Agents, Phytogenic
- Enzyme Inhibitors
- Plant Extracts
- Topoisomerase I Inhibitors
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Topics |
- Annonaceae
(chemistry)
- Antineoplastic Agents, Phytogenic
(pharmacology)
- Cell Line
- Drug Screening Assays, Antitumor
- Enzyme Inhibitors
(pharmacology)
- Humans
- Plant Extracts
(pharmacology)
- Topoisomerase I Inhibitors
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