Interleukin-1beta and tumour necrosis factor-alpha levels in periapical exudates.

Abstract

AIM:

The aim of this study was to determine IL-1beta and TNF-alpha levels in periapical exudates and to evaluate their relationship with clinical and radiological findings.

METHODOLOGY:

Periapical exudates were collected from root canals of 35 single-rooted teeth using absorbent paper points. IL-1beta and TNF-alpha levels were determined by enzyme-linked immunosorbent assays. The samples were divided into two groups according to the presence or absence of clinical signs including swelling and/or fistula, pain on palpation and/or percussion, and pus discharge from canals. Periapical exudate samples were divided into two groups according to size of periapical radiolucent area.

RESULTS:

The mean concentration of IL-1beta (72.79 ng mL-1) in periapical exudates was approximately 12-fold higher than TNF-alpha(6.17 ng mL-1). There was no significant correlation between these cytokines (P > 0.05). IL-1beta levels in canals with larger radiolucent areas (long axis > or =1 cm) were significantly higher than those with small areas (P < 0.05). There was a tendency toward higher levels of IL-1beta in groups with clinical signs, but the differences were not significant (P > 0.05).

CONCLUSIONS:

Periapical exudate levels of both cytokines failed to reflect periapical disease state.

• Authors: T Ataoğlu, M Ungör, B Serpek, S Haliloğlu, H Ataoğlu, H Ari
• Journal: International endodontic journal (Int Endod J) Vol. 35 Issue 2 Pg. 181-5 (Feb 2002) ISSN: 0143-2885 [Print] England
• PMID: 11843974 (Publication Type: Journal Article)

Chemical References

• Interleukin-1
• Tumor Necrosis Factor-alpha

Topics

• Alveolar Bone Loss (metabolism)
• Enzyme-Linked Immunosorbent Assay
• Exudates and Transudates (chemistry, metabolism)
• Humans
• Interleukin-1 (analysis, metabolism)
• Periapical Granuloma (metabolism)
• Statistics, Nonparametric
• Tumor Necrosis Factor-alpha (analysis, metabolism)