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Immunohistochemical detection of salivary agglutinin/gp-340 in human parotid, submandibular, and labial salivary glands.

Abstract
Salivary agglutinin is a Streptococcus mutans binding protein and a member of the scavenger receptor cysteine-rich superfamily. It is identical to lung gp-340 and brain DMBT1, which possibly play a role in innate immunity and tumor suppression, respectively. The goal of this study was to localize salivary agglutinin in human salivary glands. Two monoclonal antibodies, directed against gp-340, were characterized. mAb 213-1 reacted with sialic acid epitopes and cross-reacted with MUC7. The reaction with mAb 213-6 disappeared after reduction, suggesting that a protein epitope was recognized. In the parotid gland, immunohistochemical labeling with mAb 213-6 was found in the duct cells. In the submandibular gland and labial gland, both serous acini and demilune cells were labeled. In the labial gland, labeling was found at the luminal side of the duct cells. Salivary agglutinin was distinctly localized in salivary glands, but in distinct glandular secretions, no differences in electrophoretic behavior were observed.
AuthorsF J Bikker, A J M Ligtenberg, J E van der Wal, P A M van den Keijbus, U Holmskov, E C I Veerman, A V Nieuw Amerongen
JournalJournal of dental research (J Dent Res) Vol. 81 Issue 2 Pg. 134-9 (Feb 2002) ISSN: 0022-0345 [Print] United States
PMID11829014 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Antibodies, Monoclonal
  • Epitopes
  • MUC7 protein, human
  • Mucins
  • Receptors, Immunologic
  • Salivary Proteins and Peptides
  • glycoprotein 340
  • N-Acetylneuraminic Acid
Topics
  • Aged
  • Antibodies, Monoclonal
  • Blotting, Western
  • Cross Reactions (immunology)
  • Electrophoresis, Polyacrylamide Gel
  • Epitopes (immunology)
  • Female
  • Humans
  • Immunoenzyme Techniques
  • Immunohistochemistry
  • Lip (cytology, metabolism)
  • Male
  • Middle Aged
  • Mucins (analysis)
  • N-Acetylneuraminic Acid (immunology)
  • Parotid Gland (cytology, metabolism)
  • Receptors, Immunologic (analysis)
  • Saliva (chemistry)
  • Salivary Glands, Minor (cytology, metabolism)
  • Salivary Proteins and Peptides (analysis)
  • Submandibular Gland (cytology, metabolism)

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