Abstract | BACKGROUND: MATERIALS AND METHODS: We examined vascular effects of p-GlcNAc on isolated aortic rings obtained from Sprague-Dawley rats. The rings were suspended in organ baths and precontracted with U46619, a thromboxane A2 mimetic. RESULTS:
p-GlcNAc produced a concentration-dependent vasoconstriction over the range of 14 to 100 microg/ml. At a concentration of 100 microg/ml, p-GlcNAc significantly contracted aortic rings by 133 +/- 20 mg of developed force (P < 0.01). Neither a deacetylated derivative of p-GlcNAc nor a structurally related macromolecule, chitin, contracted rat aortic rings, indicating a specificity for p-GlcNAc. The vasoconstriction to p-GlcNAc was totally abolished in deendothelialized rat aortic rings, suggesting that an endothelial component is essential to the vasoconstriction. Pretreatment with the endothelin ET(A) receptor antagonist, JKC-301 (0.5 and 1 microM), significantly diminished p-GlcNAc-induced vasoconstriction by 57 to 61% (P < 0.01). However, p-GlcNAc did not significantly diminish nitric oxide release from rat aortic endothelium. CONCLUSION: These results provide evidence that p-GlcNAc significantly contracts isolated rat aortic rings via an endothelium-dependent mechanism, partly via enhancement of endothelin-1 release from endothelial cells.
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Authors | Yasuhiko Ikeda, Lindon H Young, John N Vournakis, Allan M Lefer |
Journal | The Journal of surgical research
(J Surg Res)
Vol. 102
Issue 2
Pg. 215-20
(Feb 2002)
ISSN: 0022-4804 [Print] United States |
PMID | 11796021
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
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Copyright | (c)2001 Elsevier Science. |
Chemical References |
- Enzyme Inhibitors
- Polysaccharides
- Receptors, Endothelin
- Vasoconstrictor Agents
- poly-N-acetyl glucosamine
- Nitric Oxide
- 15-Hydroxy-11 alpha,9 alpha-(epoxymethano)prosta-5,13-dienoic Acid
- NG-Nitroarginine Methyl Ester
- Acetylglucosamine
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Topics |
- 15-Hydroxy-11 alpha,9 alpha-(epoxymethano)prosta-5,13-dienoic Acid
(pharmacology)
- Acetylglucosamine
(pharmacology)
- Animals
- Aorta
(drug effects, metabolism)
- Endothelium, Vascular
(drug effects, metabolism)
- Enzyme Inhibitors
(pharmacology)
- In Vitro Techniques
- Male
- NG-Nitroarginine Methyl Ester
(pharmacology)
- Nitric Oxide
(metabolism)
- Polysaccharides
(pharmacology)
- Rats
- Rats, Sprague-Dawley
- Receptors, Endothelin
(metabolism)
- Vasoconstriction
(drug effects)
- Vasoconstrictor Agents
(pharmacology)
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