Iron chelates derived from
ethylenediaminedi(o-hydroxyphenylacetic) acid (
EDDHA),
ethylenediaminedi(o-hydroxy-p-methylphenylacetic) acid (
EDDHMA), ethylenediaminedi(2-hydroxy-5-sulfophenylacetic)
acid (EDDHSA), and ethylenediaminedi(5-carboxy-2-hydroxyphenylacetic)
acid (EDDCHA) are remarkably efficient in correcting
iron chlorosis in plants growing in alkaline
soils. This work reports the determination of impurities in commercial samples of
fertilizers containing
EDDHMA/Fe(3+), EDDHSA/Fe(3+), and EDDCHA/Fe(3+). The active components (
EDDHMA/Fe(3+), EDDHSA/Fe(3+), and EDDCHA/Fe(3+)) were separated easily from other compounds present in the
fertilizers by HPLC. Comparison of the retention times and the UV-visible spectra of the peaks obtained from commercial EDDHSA/Fe(3+) and EDDCHA/Fe(3+) samples with those of standard solutions showed that unreacted starting materials (p-hydroxybenzenesulfonic
acid and p-
hydroxybenzoic acid, respectively) were always present in the commercial products. 1D and 2D NMR experiments showed that commercial
fertilizers based on
EDDHMA/Fe(3+) contained impurities having structures tentatively assigned to
iron chelates of two isomers of
EDDHMA. These findings suggest that current production processes of
iron chelates used in agriculture need to be improved.