[Suppression of alpha-fetoprotein gene expression in somatic hybrids of AFP-positive and AFP-negative clones of rat hepatoma].

Abstract

The presented study is devoted to investigation of molecular mechanisms regulating alpha-fetoprotein (AFP) gene expression at transcriptional level. The study was carried out on AFP-positive and AFP-negative clones of rat hepatoma McA-RH 7777 that also differ in hepatocyte nuclear factors (HNF) 1 and 4 transcription levels. To examine a hypothesis of existence in AFP-non-producing clones a transcriptional factor that downregulates this gene expression, we have obtained somatic hybrids of AFP-positive and AFP-negative clones. In the obtained hybrids AFP gene expression is decreased while expression of HNF1, one of the main AFP promoter activators, is maintained. These data indicate an existence of a repressor in AFP-negative clones that determines AFP gene downregulation regardless of the HNF1 expression level.

• Authors: I F Kustova, E V Varga, T L Eraĭzer, N L Lazarevich
• Journal: Genetika (Genetika) Vol. 37 Issue 10 Pg. 1330-9 (Oct 2001) ISSN: 0016-6758 [Print] Russia (Federation)
• Vernacular Title: Podavlenie ékspressii gena al'fa-fetoproteina v somaticheskikh gibridakh AFP-polozhitel'nogo i AFP-otritsatel'nogo klonov krysinoĭ gepatomy.
• PMID: 11761608 (Publication Type: English Abstract, Journal Article)

Chemical References

• DNA-Binding Proteins
• Hepatocyte Nuclear Factor 1-alpha
• Hnf1a protein, rat
• Nuclear Proteins
• Transcription Factors
• alpha-Fetoproteins
• Hepatocyte Nuclear Factor 1
• Hepatocyte Nuclear Factor 1-beta

Topics

• Animals
• DNA-Binding Proteins
• Gene Expression Regulation
• Hepatocyte Nuclear Factor 1
• Hepatocyte Nuclear Factor 1-alpha
• Hepatocyte Nuclear Factor 1-beta
• Hybrid Cells
• Liver Neoplasms, Experimental (genetics)
• Nuclear Proteins
• Rats
• Transcription Factors (genetics)
• Transcription, Genetic (genetics)
• alpha-Fetoproteins (genetics)