CBC.37
monoclonal antibody (mAb) was generated using balb/c mice immunized with CEM T cell line. It was selected because of its strong reactivity on T lymphocytes on
paraffin tissue sections. The anti-CD7 specificity of CBC.37 mAb was assessed by immunohistochemistry, cross-blocking, and cross-immunoprecipitation experiments using CBC.37 and the anti-CD7 mAb DK24. CBC.37 mAb immunoprecipitated a 40-kDa
protein. Cross-blocking and cross-immunoprecipitation experiments demonstrated that the two
antibodies recognized the same molecule. Immunostaining of a large number of reactive lymph nodes and B and
T cell lymphomas confirms that CBC.37 mAb was directed against T cells. As expected, on reactive lymph nodes the staining pattern was comparable to that of CD3. Among the 110
T cell lymphomas examined, all T lymphoblastic
lymphomas were positive (15+/15; 100%). As a result of the frequent loss of
CD7 antigen, only 25+/95 (26%) of peripheral T cell
neoplasms were found to be positive for CBC.37. A marked reduction in the number of CBC.37-positive T cells was observed in 7 of the 60 cases of benign inflammatory
dermatoses studied (approximately 12%). CBC.37 was unreactive with all healthy and neoplastic non-lymphoid samples examined. Because the lack of CD7 expression in
T cell lymphomas is of diagnostic value, CBC.37 mAb in association with other
anti-T cell
antibodies working on
paraffin sections could be of particular value in asserting the diagnosis of
T cell lymphomas in routine histopathology.