Differentiation of resting human peripheral blood gamma delta T cells toward Th1- or Th2-phenotype.

Abstract

Depending on the microenvironment, murine gamma delta T cells differentiate into either Th1 (IFN-gamma-producing) or Th2 (IL-4-producing) cells. It is unclear, however, whether circulating human peripheral blood gamma delta T cells can be driven into Th1 or Th2 cells by modulation of the priming cytokine milieu. In this study, peripheral blood gamma delta T cells were stimulated by phosphoantigen (isopentenyl pyrophosphate) or Daudi lymphoma cells in the presence of Th1-priming (rIL-12, anti-IL-4 Ab) or Th2-priming (rIL-4, anti-IL-12 Ab) conditions. Single-cell analysis of cytokine secretion (IFN-gamma and IL-4) was performed by flow cytometry after 18 h and after restimulation of expanded gamma delta T cells. The early activation of resting gamma delta T cells was characterized by the induction of IFN-gamma. Priming under Th1 conditions induced a Th1 profile characterized by increased secretion of IFN-gamma and TNF-alpha, while Th2 conditions caused increased production of IL-4 (Th2 profile) by the gamma delta T cells. These results indicate that the major subset of human gamma delta T cells can be polarized into either Th1 or Th2 cytokine pattern depending on the cytokine milieu in which contact with antigen occurs.

• Authors: D Wesch, A Glatzel, D Kabelitz
• Journal: Cellular immunology (Cell Immunol) Vol. 212 Issue 2 Pg. 110-7 (Sep 15 2001) ISSN: 0008-8749 [Print] Netherlands
• PMID: 11748927 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antibodies, Monoclonal
• Antigens
• Hemiterpenes
• Interleukin-1
• Organophosphorus Compounds
• Receptors, Antigen, T-Cell, gamma-delta
• Recombinant Proteins
• Interleukin-12
• Interleukin-4
• isopentenyl pyrophosphate
• Ionomycin
• Interferon-gamma
• Tetradecanoylphorbol Acetate

Topics

• Antibodies, Monoclonal (immunology, pharmacology)
• Antigens (immunology)
• Burkitt Lymphoma (pathology)
• Cell Differentiation (drug effects)
• Cell Survival
• Cells, Cultured
• Flow Cytometry
• Hemiterpenes
• Humans
• Immunophenotyping
• Interferon-gamma (metabolism)
• Interleukin-1 (pharmacology)
• Interleukin-12 (pharmacology)
• Interleukin-4 (metabolism, pharmacology)
• Ionomycin (pharmacology)
• Lymphocyte Activation
• Organophosphorus Compounds (immunology)
• Receptors, Antigen, T-Cell, gamma-delta (immunology)
• Recombinant Proteins (pharmacology)
• T-Lymphocyte Subsets (chemistry, cytology, drug effects)
• Tetradecanoylphorbol Acetate (pharmacology)
• Th1 Cells (cytology)
• Th2 Cells (cytology)
• Tumor Cells, Cultured