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Calcium puffs are generic InsP(3)-activated elementary calcium signals and are downregulated by prolonged hormonal stimulation to inhibit cellular calcium responses.

Abstract
Elementary Ca(2+) signals, such as "Ca(2+) puffs", which arise from the activation of inositol 1,4,5-trisphosphate receptors, are building blocks for local and global Ca(2+) signalling. We characterized Ca(2+) puffs in six cell types that expressed differing ratios of the three inositol 1,4,5-trisphosphate receptor isoforms. The amplitudes, spatial spreads and kinetics of the events were similar in each of the cell types. The resemblance of Ca(2+) puffs in these cell types suggests that they are a generic elementary Ca(2+) signal and, furthermore, that the different inositol 1,4,5-trisphosphate isoforms are functionally redundant at the level of subcellular Ca(2+) signalling. Hormonal stimulation of SH-SY5Y neuroblastoma cells and HeLa cells for several hours downregulated inositol 1,4,5-trisphosphate expression and concomitantly altered the properties of the Ca(2+) puffs. The amplitude and duration of Ca(2+) puffs were substantially reduced. In addition, the number of Ca(2+) puff sites active during the onset of a Ca(2+) wave declined. The consequence of the changes in Ca(2+) puff properties was that cells displayed a lower propensity to trigger regenerative Ca(2+) waves. Therefore, Ca(2+) puffs underlie inositol 1,4,5-trisphosphate signalling in diverse cell types and are focal points for regulation of cellular responses.
AuthorsS C Tovey, P de Smet, P Lipp, D Thomas, K W Young, L Missiaen, H De Smedt, J B Parys, M J Berridge, J Thuring, A Holmes, M D Bootman
JournalJournal of cell science (J Cell Sci) Vol. 114 Issue Pt 22 Pg. 3979-89 (Nov 2001) ISSN: 0021-9533 [Print] England
PMID11739630 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Calcium Channels
  • ITPR1 protein, human
  • Inositol 1,4,5-Trisphosphate Receptors
  • Protein Isoforms
  • Receptors, Cytoplasmic and Nuclear
  • Histamine
  • Inositol 1,4,5-Trisphosphate
  • Adenosine Triphosphate
  • Carbachol
  • Calcium
Topics
  • Adenosine Triphosphate (pharmacology)
  • Animals
  • Calcium (metabolism)
  • Calcium Channels (genetics, metabolism)
  • Calcium Signaling (drug effects, physiology)
  • Carbachol (pharmacology)
  • Cell Line
  • Cell Nucleus (metabolism)
  • Down-Regulation
  • Histamine (pharmacology)
  • Humans
  • Immunohistochemistry
  • Inositol 1,4,5-Trisphosphate (metabolism)
  • Inositol 1,4,5-Trisphosphate Receptors
  • Protein Isoforms (genetics, metabolism)
  • Receptors, Cytoplasmic and Nuclear (genetics, metabolism)

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