Immunization with a portion of rickettsial outer membrane protein A stimulates protective immunity against spotted fever rickettsiosis.

Abstract

Two approaches for presentation of a part of the rickettsial outer membrane protein A (OmpA) of Rickettsia rickettsii, namely (1) recombinant Mycobacterium vaccae (rMV) or (2) recombinant DNA vaccine, stimulated protective immunity against a lethal challenge with the closely related bacterium, R. conorii, in mice. After primary immunization with rMV and booster immunization with homologous recombinant protein, 67 and 55% of mice were protected against challenge in two experiments. DNA vaccination with booster recombinant protein immunization protected six out of eight animals from a lethal challenge. Production of IFN-gamma by antigen-exposed T-lymphocytes of DNA vaccine recipients indicated that cellular immunity had been stimulated.

• Authors: P A Crocquet-Valdes, C M Díaz-Montero, H M Feng, H Li, A D Barrett, D H Walker
• Journal: Vaccine (Vaccine) Vol. 20 Issue 5-6 Pg. 979-88 (Dec 12 2001) ISSN: 0264-410X [Print] Netherlands
• PMID: 11738766 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Bacterial Outer Membrane Proteins
• DNA, Bacterial
• Recombinant Fusion Proteins
• Rickettsial Vaccines
• Vaccines, DNA
• Interferon-gamma

Topics

• Animals
• Bacterial Outer Membrane Proteins (genetics, immunology)
• Base Sequence
• DNA, Bacterial (genetics)
• Escherichia coli (genetics)
• Immunization, Secondary
• Interferon-gamma (biosynthesis)
• Male
• Mice
• Mice, Inbred C3H
• Mycobacterium (genetics)
• Recombinant Fusion Proteins (genetics, immunology)
• Rickettsia rickettsii (genetics, immunology)
• Rickettsial Vaccines (genetics, pharmacology)
• Rocky Mountain Spotted Fever (immunology, prevention & control)
• T-Lymphocytes (immunology)
• Transformation, Genetic
• Vaccines, DNA (genetics, pharmacology)