Abstract | BACKGROUND: From the observations of morphology seen in early phases of the experimental models of the irreversible mesangial proliferative glomerulonephritis, we hypothesized that podocyte injury is one of the important factors in bringing upon irreversible glomerular alterations. To verify this hypothesis, we investigated whether podocyte injury induced by puromycin aminonucleoside (PAN) injection affects the mesangial alterations of anti-Thy 1.1 glomerulonephritis. METHODS: RESULTS: Consecutive injections of 10 mg/100 g BW of PAN and mAb 1-22-3 caused the irreversible mesangial alteration with persistent proteinuria (at week 8, proteinuria 100.3 +/- 57.8 mg/24 h, matrix score 1.13 +/- 0.52, collagen type I score 2.04 +/- 0.53, mRNA for collagen type I 227 +/- 79% to the group with a single injection of 1-22-3). Although single injection of 5 mg/100 g BW of PAN was not capable of inducing abnormal proteinuria, consecutive injections of 5 mg/100 g BW of PAN and mAb 1-22-3 also caused irreversible mesangial alteration and persistent proteinuria. CONCLUSIONS: Podocyte injury might be an important factor that exacerbates mesangial proliferation and mesangial matrix expansion. The irreversible mesangial alterations caused by consecutive injections of PAN and mAb 1-22-3 may be a novel model that could be used to analyze the mechanism of progressive mesangial alteration.
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Authors | Y Morioka, H Koike, Y Ikezumi, Y Ito, A Oyanagi, F Gejyo, F Shimizu, H Kawachi |
Journal | Kidney international
(Kidney Int)
Vol. 60
Issue 6
Pg. 2192-204
(Dec 2001)
ISSN: 0085-2538 [Print] United States |
PMID | 11737593
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antibodies, Monoclonal
- Thy-1 Antigens
- Puromycin Aminonucleoside
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Topics |
- Animals
- Antibodies, Monoclonal
(pharmacology)
- Female
- Glomerular Mesangium
(drug effects, pathology)
- Glomerulonephritis, Membranoproliferative
(chemically induced, pathology)
- Kidney
(pathology)
- Puromycin Aminonucleoside
(pharmacology)
- Rats
- Rats, Wistar
- Thy-1 Antigens
(immunology)
- Time Factors
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